ArticleMolecular biology of the cell2001
Uptake of the ATP-binding cassette (ABC) transporter Ste6 into the yeast vacuole is blocked in the doa4 Mutant.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4.Traffic (Copenhagen, Denmark) · 2022Article
- Vacuolar H+-ATPase dysfunction rescues intralumenal vesicle cargo sorting in yeast lacking PI(3,5)P2 or Doa4.Journal of cell science · 2021Article
- Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase.Molecular biology of the cell · 2017Article
- Biogenesis of the Saccharomyces cerevisiae pheromone a-factor, from yeast mating to human disease.Microbiology and molecular biology reviews : MMBR · 2012Review
- Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity.The Journal of biological chemistry · 2011Article
- Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.Journal of virology · 2011Article
- Bro1 binding to Snf7 regulates ESCRT-III membrane scission activity in yeast.The Journal of cell biology · 2011Article
- The ataxia (axJ) mutation causes abnormal GABAA receptor turnover in mice.PLoS genetics · 2009Article
- Membrane protein targeting to the MVB/lysosome.Chemical reviews · 2009Review
- DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies.The Journal of biological chemistry · 2008Article
- Article
- Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.The EMBO journal · 2007Article
- Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway.Molecular biology of the cell · 2007Article
- Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes.Molecular biology of the cell · 2005Article
- Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- Control of Ste6 recycling by ubiquitination in the early endocytic pathway in yeast.Molecular biology of the cell · 2005Article
- The deubiquitinating enzyme Ubp1 affects sorting of the ATP-binding cassette-transporter Ste6 in the endocytic pathway.Molecular biology of the cell · 2005Article
- Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans.Molecular biology of the cell · 2004Article
- Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes.The Journal of cell biology · 2004Article
- VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments.The Journal of cell biology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous experiments suggested that trafficking of the a-factor transporter Ste6 of Saccharomyces cerevisiae to the yeast vacuole is regulated by ubiquitination. To define the ubiquitination-dependent step in the trafficking pathway, we examined the intracellular localization of Ste6 in the ubiquitination-deficient doa4 mutant by immunofluorescence experiments, with a Ste6-green fluorescent protein fusion protein and by sucrose density gradient fractionation. We found that Ste6 accumulated at the vacuolar membrane in the doa4 mutant and not at the cell surface. Experiments with a doa4 pep4 double mutant showed that Ste6 uptake into the lumen of the vacuole is inhibited in the doa4 mutant. The uptake defect could be suppressed by expression of additional ubiquitin, indicating that it is primarily the result of a lowered ubiquitin level (and thus of reduced ubiquitination) and not the result of a deubiquitination defect. Based on our findings, we propose that ubiquitination of Ste6 or of a trafficking factor is required for Ste6 sorting into the multivesicular bodies pathway. In addition, we obtained evidence suggesting that Ste6 recycles between an internal compartment and the plasma membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.