ArticleMolecular biology of the cell2001
Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked or inhibited in rat tissues.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- The expression of essential selenoproteins during development requires SECIS-binding protein 2-like.Life science alliance · 2022Article
- Identification of Selenoprotein H Isoforms and Impact of Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2 Other Selenoproteins in 293T Cells.The Journal of nutrition · 2021Article
- Article
- The RNA-binding protein Secisbp2 differentially modulates UGA codon reassignment and RNA decay.Nucleic acids research · 2017Article
- Modeling and gene knockdown to assess the contribution of nonsense-mediated decay, premature termination, and selenocysteine insertion to the selenoprotein hierarchy.RNA (New York, N.Y.) · 2016Article
- In Silico Analysis of the Structural and Biochemical Features of the NMD Factor UPF1 in Ustilago maydis.PloS one · 2016Article
- SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.Science (New York, N.Y.) · 2015Article
- Nonsense-mediated decay factors are involved in the regulation of selenoprotein mRNA levels during selenium deficiency.RNA (New York, N.Y.) · 2014Article
- Hypermethylated-capped selenoprotein mRNAs in mammals.Nucleic acids research · 2014Article
- Selective up-regulation of human selenoproteins in response to oxidative stress.The Journal of biological chemistry · 2014Article
- Article
- Glutathione peroxidase-1 in health and disease: from molecular mechanisms to therapeutic opportunities.Antioxidants & redox signaling · 2011Review
- Selenium regulation of the selenoprotein and nonselenoprotein transcriptomes in rodents.Advances in nutrition (Bethesda, Md.) · 2011Review
- mRNA degradation controls differentiation state-dependent differences in transcript and splice variant abundance.Nucleic acids research · 2011Article
- Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors.Cellular and molecular life sciences : CMLS · 2010Review
- Mouse models targeting selenocysteine tRNA expression for elucidating the role of selenoproteins in health and development.Molecules (Basel, Switzerland) · 2009Review
- Selenium status highly regulates selenoprotein mRNA levels for only a subset of the selenoproteins in the selenoproteome.Bioscience reports · 2009Article
- Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development.Genes & development · 2008Article
- The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply.Nucleic acids research · 2007Article
- Nuclear assembly of UGA decoding complexes on selenoprotein mRNAs: a mechanism for eluding nonsense-mediated decay?Molecular and cellular biology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies of mRNA for classical glutathione peroxidase 1 (GPx1) demonstrated that hepatocytes of rats fed a selenium-deficient diet have less cytoplasmic GPx1 mRNA than hepatocytes of rats fed a selenium-adequate diet. This is because GPx1 mRNA is degraded by the surveillance pathway called nonsense-mediated mRNA decay (NMD) when the selenocysteine codon is recognized as nonsense. Here, we examine the mechanism by which the abundance of phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA, another selenocysteine-encoding mRNA, fails to decrease in the hepatocytes and testicular cells of rats fed a selenium-deficient diet. We demonstrate with cultured NIH3T3 fibroblasts or H35 hepatocytes transiently transfected with PHGPx gene variants under selenium-supplemented or selenium-deficient conditions that PHGPx mRNA is, in fact, a substrate for NMD when the selenocysteine codon is recognized as nonsense. We also demonstrate that the endogenous PHGPx mRNA of untransfected H35 cells is subject to NMD. The failure of previous reports to detect the NMD of PHGPx mRNA in cultured cells is likely attributable to the expression of PHGPx cDNA rather than the PHGPx gene. We conclude that 1) the sequence of the PHGPx gene is adequate to support the NMD of product mRNA, and 2) there is a mechanism in liver and testis but not cultured fibroblasts and hepatocytes that precludes or masks the NMD of PHGPx mRNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.