ArticleMolecular biology of the cell2001
Identification of discrete classes of endosome-derived small vesicles as a major cellular pool for recycling membrane proteins.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Vesicular Trafficking to the Immune Synapse: How to Assemble Receptor-Tailored Pathways from a Basic Building Set.Frontiers in immunology · 2016Review
- Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm.Open biology · 2015Article
- Specific recycling receptors are targeted to the immune synapse by the intraflagellar transport system.Journal of cell science · 2014Article
- Differential subcellular distribution of endosomal compartments and the dopamine transporter in dopaminergic neurons.Molecular and cellular neurosciences · 2011Article
- Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- A fluorescence-based in vitro assay for investigating early endosome dynamics.Nature protocols · 2010Article
- Sorting in early endosomes reveals connections to docking- and fusion-associated factors.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mutation of the membrane-associated M1 protease APM1 results in distinct embryonic and seedling developmental defects in Arabidopsis.The Plant cell · 2009Article
- Involvement of insulin-regulated aminopeptidase in the effects of the renin-angiotensin fragment angiotensin IV: a review.Heart failure reviews · 2008Review
- Down syndrome fibroblast model of Alzheimer-related endosome pathology: accelerated endocytosis promotes late endocytic defects.The American journal of pathology · 2008Article
- Translocation of the insulin-regulated aminopeptidase to the cell surface: detection by radioligand binding.British journal of pharmacology · 2008Article
- Effect of CCR5 receptor antagonists on endocytosis of the human CCR5 receptor in CHO-K1 cells.British journal of pharmacology · 2008Article
- Ins (endocytosis) and outs (exocytosis) of GLUT4 trafficking.Current opinion in cell biology · 2007Review
- Endocytic trafficking of CFTR in health and disease.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2007Review
- Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking.Diabetologia · 2005Article
- Identification of 80K-H as a protein involved in GLUT4 vesicle trafficking.The Biochemical journal · 2005Article
- Localization of brain endothelial luminal and abluminal transporters with immunogold electron microscopy.NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics · 2005Review
- In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5.Molecular biology of the cell · 2004Article
- Elevated endosomal cholesterol levels in Niemann-Pick cells inhibit rab4 and perturb membrane recycling.Molecular biology of the cell · 2004Article
- Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Vesicles carrying recycling plasma membrane proteins from early endosomes have not yet been characterized. Using Chinese hamster ovary cells transfected with the facilitative glucose transporter, GLUT4, we identified two classes of discrete, yet similarly sized, small vesicles that are derived from early endosomes. We refer to these postendosomal vesicles as endocytic small vesicles or ESVs. One class of ESVs contains a sizable fraction of the pool of the transferrin receptor, and the other contains 40% of the total cellular pool of GLUT4 and is enriched in the insulin-responsive aminopeptidase (IRAP). The ESVs contain cellubrevin and Rab4 but are lacking other early endosomal markers, such as EEA1 or syntaxin13. The ATP-, temperature-, and cytosol-dependent formation of ESVs has been reconstituted in vitro from endosomal membranes. Guanosine 5'-[gamma-thio]triphosphate and neomycin, but not brefeldin A, inhibit budding of the ESVs in vitro. A monoclonal antibody recognizing the GLUT4 cytoplasmic tail perturbs the in vitro targeting of GLUT4 to the ESVs without interfering with the incorporation of IRAP or TfR. We suggest that cytosolic proteins mediate the incorporation of recycling membrane proteins into discrete populations of ESVs that serve as carrier vesicles to store and then transport the cargo from early endosomes, either directly or indirectly, to the cell surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.