ArticleMolecular biology of the cell2001
Sequence-specific interaction between the disintegrin domain of mouse ADAM 3 and murine eggs: role of beta1 integrin-associated proteins CD9, CD81, and CD98.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 124 citations in OpenAlex.
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- A Comparative Review of Fertility and Semen Assessment Techniques in Farm Animals.Animals : an open access journal from MDPI · 2026Review
- Article
- Where are all the egg genes?Frontiers in cell and developmental biology · 2023Review
- Phenotype Compensation in Reproductive ADAM Gene Family: A Case Study with ADAM27 Knockout Mouse.Iranian journal of biotechnology · 2022Article
- Review
- Sperm-oocyte signaling: the role of IZUMO1R and CD9 in PTK2B activation and actin remodeling at the sperm binding site†.Biology of reproduction · 2021Article
- Oolemma Receptors in Mammalian Molecular Fertilization: Function and New Methods of Study.Frontiers in cell and developmental biology · 2021Review
- Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos.Medical microbiology and immunology · 2020Review
- A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis.Medical microbiology and immunology · 2020Article
- The molecular basis of fertilization (Review).International journal of molecular medicine · 2016Review
- Review
- Critical role of exosomes in sperm-egg fusion and virus-induced cell-cell fusion.Reproductive medicine and biology · 2013Review
- Testicular and epididymal ADAMs: expression and function during fertilization.Nature reviews. Urology · 2012Review
- Evolution of genes involved in gamete interaction: evidence for positive selection, duplications and losses in vertebrates.PloS one · 2012Article
- Functional interplay between tetraspanins and proteases.Cellular and molecular life sciences : CMLS · 2011Review
- The sheddase activity of ADAM17/TACE is regulated by the tetraspanin CD9.Cellular and molecular life sciences : CMLS · 2011Article
- Immunohistochemical distribution of the tetraspanin CD9 in normal porcine tissues.Molecular biology reports · 2011Article
- Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
ADAM 3 is a sperm surface glycoprotein that has been implicated in sperm-egg adhesion. Because little is known about the adhesive activity of ADAMs, we investigated the interaction of ADAM 3 disintegrin domains, made in bacteria and in insect cells, with murine eggs. Both recombinant proteins inhibited sperm-egg binding and fusion with potencies similar to that which we recently reported for the ADAM 2 disintegrin domain. Alanine scanning mutagenesis revealed a critical importance for the glutamine at position 7 of the disintegrin loop. Fluorescent beads coated with the ADAM 3 disintegrin domain bound to the egg surface. Bead binding was inhibited by an authentic, but not by a scrambled, peptide analog of the disintegrin loop. Bead binding was also inhibited by the function-blocking anti-alpha6 monoclonal antibody (mAb) GoH3, but not by a nonfunction blocking anti-alpha6 mAb, or by mAbs against either the alphav or beta3 integrin subunits. We also present evidence that in addition to the tetraspanin CD9, two other beta1-integrin-associated proteins, the tetraspanin CD81 as well as the single pass transmembrane protein CD98 are expressed on murine eggs. Antibodies to CD9 and CD98 inhibited in vitro fertilization and binding of the ADAM 3 disintegrin domain. Our findings are discussed in terms of the involvement of multiple sperm ADAMs and multiple egg beta1 integrin-associated proteins in sperm-egg binding and fusion. We propose that an egg surface "tetraspan web" facilitates fertilization and that it may do so by fostering ADAM-integrin interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.