ArticleNucleic acids research2001
Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.Molecular cancer · 2022Article
- A statistical approach for 5' splice site prediction using short sequence motifs and without encoding sequence data.BMC bioinformatics · 2014Article
- Human genetic variation recognizes functional elements in noncoding sequence.Genome research · 2010Article
- Large-scale comparative analysis of splicing signals and their corresponding splicing factors in eukaryotes.Genome research · 2008Article
- DMS footprinting of structured RNAs and RNA-protein complexes.Nature protocols · 2007Article
- Dichotomous splicing signals in exon flanks.Genome research · 2005Article
- The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex.Molecular and cellular biology · 2003Article
- The ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5' to the branch site.RNA (New York, N.Y.) · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Three different base paired stems form between U2 and U6 snRNA over the course of the mRNA splicing reaction (helices I, II and III). One possible function of U2/U6 helix II is to facilitate subsequent U2/U6 helix I and III interactions, which participate directly in catalysis. Using an in vitro trans-splicing assay, we investigated the function of sequences located just upstream from the branch site (BS). We find that these upstream sequences are essential for stable binding of U2 to the branch region, and for U2/U6 helix II formation, but not for initial U2/BS pairing. We also show that non-functional upstream sequences cause U2 snRNA stem-loop IIa to be exposed to dimethylsulfate modification, perhaps reflecting a U2 snRNA conformational change and/or loss of SF3b proteins. Our data suggest that initial binding of U2 snRNP to the BS region must be stabilized by an interaction with upstream sequences before U2/U6 helix II can form or U2 stem-loop IIa can participate in spliceosome assembly.
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Registered trials
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