ArticleInfection and immunity2001
Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed.
- The shared evolutionary capacities of plasmids and extrachromosomal DNA.Nature reviews. Genetics · 2026Review
- Lithocholic acid induces T3SS-dependent formation of invasion-competentInfection and immunity · 2026Article
- The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneri.Nucleic acids research · 2026Article
- The pcnB gene sustains Shigella flexneri virulence.PLoS pathogens · 2025Article
- Fecal microbiome profiling of children withMicrobiology spectrum · 2025Article
- H-NS is a bacterial transposon capture protein.Nature communications · 2024Article
- The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression.Nature communications · 2024Article
- Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Novel inhibitors that target bacterial virulence identified via HTS against intra-macrophage survival ofmSphere · 2023Article
- Engineered Escherichia coli for the in situ secretion of therapeutic nanobodies in the gut.Cell host & microbe · 2023Article
- Novel plasmids in multidrug-resistant Shigella flexneri serotypes from Pakistan.Archives of microbiology · 2023Article
- Toward aInternational journal of molecular sciences · 2023Review
- The AraC/XylS Protein MxiE and Its Coregulator IpgC Control a Negative Feedback Loop in the Transcriptional Cascade That Regulates Type III Secretion in Shigella flexneri.Journal of bacteriology · 2022Article
- Virulence plasmid pINV as a genetic signature forMicrobial genomics · 2022Article
- A Highly Unstable and Elusive Plasmid That Encodes the Type III Secretion System Is Necessary for Full Virulence in the Marine Fish PathogenInternational journal of molecular sciences · 2022Article
- Maintenance of the Shigella sonnei Virulence Plasmid Is Dependent on Its Repertoire and Amino Acid Sequence of Toxin-Antitoxin Systems.Journal of bacteriology · 2022Article
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- Four single-basepair mutations in the ptx promoter of Bordetella bronchiseptica are sufficient to activate the expression of pertussis toxin.Scientific reports · 2021Article
- The T3SS ofMicroorganisms · 2020Review
27 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
The complete sequence analysis of the 210-kb Shigella flexneri 5a virulence plasmid was determined. Shigella spp. cause dysentery and diarrhea by invasion and spread through the colonic mucosa. Most of the known Shigella virulence determinants are encoded on a large plasmid that is unique to virulent strains of Shigella and enteroinvasive Escherichia coli; these known genes account for approximately 30 to 35% of the virulence plasmid. In the complete sequence of the virulence plasmid, 286 open reading frames (ORFs) were identified. An astonishing 153 (53%) of these were related to known and putative insertion sequence (IS) elements; no known bacterial plasmid has previously been described with such a high proportion of IS elements. Four new IS elements were identified. Fifty putative proteins show no significant homology to proteins of known function; of these, 18 have a G+C content of less than 40%, typical of known virulence genes on the plasmid. These 18 constitute potentially unknown virulence genes. Two alleles of shet2 and five alleles of ipaH were also identified on the plasmid. Thus, the plasmid sequence suggests a remarkable history of IS-mediated acquisition of DNA across bacterial species. The complete sequence will permit targeted characterization of potential new Shigella virulence determinants.
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