ArticleInfection and immunity2001
Stable transfection of the bovine NRAMP1 gene into murine RAW264.7 cells: effect on Brucella abortus survival.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 101 citations in OpenAlex.
- Association Analysis ofAnimals : an open access journal from MDPI · 2025Article
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- Molecular Cloning and Functional Characterization of the 5' Regulatory Region of theAnimals : an open access journal from MDPI · 2023Article
- Seroprevalence, hematological and biochemical alterations in Brucella-seropositive Muturu cattle in Nigeria.Veterinary research communications · 2022Article
- Establishment of an Efficient Immortalization Strategy Using HMEJ-Based bInternational journal of molecular sciences · 2021Article
- Hub Proteins Involved in RAW 264.7 Macrophages Exposed to Direct Current Electric Field.International journal of molecular sciences · 2020Article
- Loci Associated With Antibody Response in Feral Swine (Frontiers in veterinary science · 2020Article
- Seroprevalence of brucellosis and associated factors among livestock slaughtered in Oko-Oba abattoir, Lagos State, southwestern Nigeria.The Pan African medical journal · 2020Article
- Quantification and comparison of TLR2 activity in monocyte-derived macrophages of zebu and crossbred cattle.Iranian journal of veterinary research · 2018Article
- Evaluation of the association of SLC11A1 gene polymorphism with incidence of paratuberculosis in goats.Journal of genetics · 2017Article
- Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects.Genome biology · 2017Article
- Prevalence of bovine brucellosis in slaughtered cattle and barriers to better protection of abattoir workers in Ibadan, South-Western Nigeria.The Pan African medical journal · 2017Article
- Examining the role of components of Slc11a1 (Nramp1) in the susceptibility of New Zealand sea lions (Phocarctos hookeri) to disease.PloS one · 2015Article
- Nitric oxide not apoptosis mediates differential killing of Mycobacterium bovis in bovine macrophages.PloS one · 2013Article
- Genetic analysis of the 3' untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms.Molecular biology reports · 2013Article
- A large seroprevalence survey of brucellosis in cattle herds under diverse production systems in northern Nigeria.BMC veterinary research · 2012Article
- Comparative analysis of the early transcriptome of Brucella abortus--infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis.Research in veterinary science · 2011Article
- Effect of polymorphisms in the Slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two Bos breeds (Blanco Orejinegro and Zebu).Genetics and molecular biology · 2010Article
- Precision genetics for complex objectives in animal agriculture.Journal of animal science · 2010Article
- A critical analysis of disease-associated DNA polymorphisms in the genes of cattle, goat, sheep, and pig.Mammalian genome : official journal of the International Mammalian Genome Society · 2008Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resistance- and susceptibility-associated alleles of bovine NRAMP1 as stable transgenes under the regulatory control of the bovine NRAMP1 promoter in the murine RAW264.7 macrophage cell line (Bcg(s)) to analyze the regulation of the NRAMP1 gene and its role in macrophage function. We demonstrated that the 5'-flanking region of bovine NRAMP1, despite the lack of TATA and CAAT boxes, has a functional promoter capable of driving the expression of a transgene in murine macrophages. A polymorphism within a microsatellite in the 3' untranslated region critically affects the expression of bovine NRAMP1 and the control of in vitro replication of Brucella abortus but not Salmonella enterica serovar Dublin. We did not observe any differences in the production of NO by resting or gamma interferon (IFN-gamma)- and IFN-gamma-lipopolysaccharide (LPS)-treated transfected cell lines, yet the resistant transfected cell lines produced significantly less NO than other cell lines, following stimulation with LPS at 24 and 48 h.
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