ArticleGenetics2001
Regulation of activator/dissociation transposition by replication and DNA methylation.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase.Science (New York, N.Y.) · 2025Article
- Near-complete telomere-to-telomere de novo genome assembly in Egyptian clover (Trifolium alexandrinum).DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Jump-starting life: balancing transposable element co-option and genome integrity in the developing mammalian embryo.EMBO reports · 2024Review
- From parasites to partners: exploring the intricacies of host-transposon dynamics and coevolution.Functional & integrative genomics · 2023Review
- Transposase expression, element abundance, element size, and DNA repair determine the mobility and heritability ofFrontiers in cell and developmental biology · 2023Article
- Toward the development of Ac/Ds transposon-mediated gene tagging system for functional genomics in oat (Avena sativa L.).Functional & integrative genomics · 2022Article
- DNA transposons mediate duplications via transposition-independent and -dependent mechanisms in metazoans.Nature communications · 2021Article
- A Field Guide to Eukaryotic Transposable Elements.Annual review of genetics · 2020Review
- Small RNA-MediatedGenetics · 2020Article
- Review
- The genomic proliferation of transposable elements in colonizing populations: Schistosoma mansoni in the new world.Genetica · 2015Article
- Methylation of microRNA genes regulates gene expression in bisexual flower development in andromonoecious poplar.Journal of experimental botany · 2015Article
- Genomic landscape of human, bat, and ex vivo DNA transposon integrations.Molecular biology and evolution · 2014Article
- DNA transposon-based gene vehicles - scenes from an evolutionary drive.Journal of biomedical science · 2013Review
- Generation of tandem direct duplications by reversed-ends transposition of maize ac elements.PLoS genetics · 2013Article
- McClintock's challenge in the 21st century.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Ac/Ds-induced chromosomal rearrangements in rice genomes.Mobile genetic elements · 2012Article
- Mutagenesis of barley malting quality QTLs with Ds transposons.Functional & integrative genomics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids ("chromatid selectivity"). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethylated Ds is unable to excise from a nonreplicating vector and that replication restores excision. A Ds element hemi-methylated on one DNA strand transposes in the absence of replication, whereas hemi-methylation of the complementary strand causes a >6.3-fold inhibition of Ds excision. Consistently in the active hemi-methylated state, the Ds ends have a high binding affinity for the transposase, whereas binding to inactive ends is strongly reduced. These results provide strong evidence for the above-mentioned model. Moreover, in the absence of DNA methylation, replication enhances Ds transposition in petunia protoplasts >8-fold and promotes formation of a predominant excision footprint. Accordingly, replication also has a methylation-independent regulatory effect on transposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.