Evidence map›Paper›PMID 11290706›Full record

ArticleGenetics2001

The spindle checkpoint of the yeast Saccharomyces cerevisiae requires kinetochore function and maps to the CBF3 domain.

R D Gardner, A Poddar, C Yellman, P A Tavormina, M C Monteagudo, D J Burke

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 50 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Generating chromosome instability through the simultaneous deletion of Mad2 and p53.Proceedings of the National Academy of Sciences of the United States of America · 2005
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

R D GardnerDepartment of Biochemistry and Molecular Genetics, University of Virginia Medical Center, University of Virginia, Charlottesville, Virginia 22908-0733, USA.
A Poddar
C Yellman
P A Tavormina
M C Monteagudo
D J Burke
University of Virginia Medical Center · USUniversity of Oklahoma Health Sciences Center · US

Funding

GENETIC CONTROL OF MITOTIC SPINDLE ASSEMBLY AND FUNCTIONR01GM040334 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI BURKE, DANIEL J · 1993 to 2005
$1.4M
GENETIC ANALYSIS OF REGULATED TUBULIN SYNTHESISR29GM040334 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI BURKE, DANIEL J · 1988 to 1993
–
NIGMS NIH HHS GM-40334
6 · The paper itself

Abstract

We have measured the activity of the spindle checkpoint in null mutants lacking kinetochore activity in the yeast Saccharomyces cerevisiae. We constructed deletion mutants for nonessential genes by one-step gene replacements. We constructed heterozygous deletions of one copy of essential genes in diploid cells and purified spores containing the deletion allele. In addition, we made gene fusions for three essential genes to target the encoded proteins for proteolysis (degron alleles). We determined that Ndc10p, Ctf13p, and Cep3p are required for checkpoint activity. In contrast, cells lacking Cbf1p, Ctf19p, Mcm21p, Slk19p, Cse4p, Mif2p, Mck1p, and Kar3p are checkpoint proficient. We conclude that the kinetochore plays a critical role in checkpoint signaling in S. cerevisiae. Spindle checkpoint activity maps to a discreet domain within the kinetochore and depends on the CBF3 protein complex.

Indexed as

Cell Cycle ProteinsMicrotubule-Associated ProteinsSaccharomyces cerevisiae ProteinsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsBinding SitesChromatinChromosomal Proteins, Non-HistoneChromosome MappingDNA-Binding ProteinsFungal ProteinsGlycogen Synthase Kinase 3KinetochoresMutagenesisNuclear ProteinsProtein-Tyrosine KinasesSaccharomyces cerevisiaeBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCBF1 protein, S cerevisiaeCBF2 protein, S cerevisiaeCell Cycle ProteinsCEP3 protein, S cerevisiaeChromatinChromosomal Proteins, Non-HistoneCSE4 protein, S cerevisiaeCTF13 protein, S cerevisiaeDNA-Binding ProteinsFungal ProteinsGlycogen Synthase Kinase 3KAR3 protein, S cerevisiaeMCK1 protein, S cerevisiaeMCM21 protein, S cerevisiaeMicrotubule-Associated ProteinsMIF2 protein, S cerevisiaeNuclear ProteinsProtein-Tyrosine KinasesSaccharomyces cerevisiae Proteins

Identifiers

PMID11290706
PMCPMC1461604
OpenAlexW2131652094

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.