ArticleMolecular and cellular biology2001
High-mobility-group proteins NHP6A and NHP6B participate in activation of the RNA polymerase III SNR6 gene.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Enhancing prime editing by fusing polymerase substrate-binding proteins to reverse transcriptase.Nucleic acids research · 2026Article
- The Non-Histone Protein FgNhp6 Is Involved in the Regulation of the Development, DON Biosynthesis, and Virulence ofPathogens (Basel, Switzerland) · 2024Article
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
- Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly.Molecular cell · 2023Article
- The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p.Scientific reports · 2022Article
- Article
- Structural Features of the Nucleosomal DNA Modulate the Functional Binding of a Transcription Factor and Productive Transcription.Frontiers in genetics · 2022Article
- The HMGB chromatin protein Nhp6A can bypass obstacles when traveling on DNA.Nucleic acids research · 2020Article
- Article
- Cloning, purification, crystallization and preliminary X-ray studies of HMO2 from Saccharomyces cerevisiae.Acta crystallographica. Section F, Structural biology communications · 2014Article
- MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.PloS one · 2012Article
- Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes.Nucleic acids research · 2011Article
- The yeast high mobility group protein HMO2, a subunit of the chromatin-remodeling complex INO80, binds DNA ends.Nucleic acids research · 2009Article
- Chromatin structure and expression of a gene transcribed by RNA polymerase III are independent of H2A.Z deposition.Molecular and cellular biology · 2008Article
- Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Article
- Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex.Molecular and cellular biology · 2004Article
- TATA-binding protein mutants that are lethal in the absence of the Nhp6 high-mobility-group protein.Molecular and cellular biology · 2004Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Transcription of yeast class III genes involves the formation of a transcription initiation complex that comprises RNA polymerase III (Pol III) and the general transcription factors TFIIIB and TFIIIC. Using a genetic screen for positive regulators able to compensate for a deficiency in a promoter element of the SNR6 gene, we isolated the NHP6A and NHP6B genes. Here we show that the high-mobility-group proteins NHP6A and NHP6B are required for the efficient transcription of the SNR6 gene both in vivo and in vitro. The transcripts of wild-type and promoter-defective SNR6 genes decreased or became undetectable in an nhp6ADelta nhp6BDelta double-mutant strain, and the protection over the TATA box of the wild-type SNR6 gene was lost in nhp6ADelta nhp6BDelta cells at 37 degrees C. In vitro, NHP6B specifically stimulated the transcription of SNR6 templates up to fivefold in transcription assays using either cell nuclear extracts from nhp6ADelta nhp6BDelta cells or reconstituted transcription systems. Finally, NHP6B activated SNR6 transcription in a TFIIIC-independent assay. These results indicate that besides the general transcription factors TFIIIB and TFIIIC, additional auxillary factors are required for the optimal transcription of at least some specific Pol III genes.
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