ArticleThe Journal of cell biology2001
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 86 citations in OpenAlex.
- Mitotic protein kinase-driven crosstalk of machineries for mitosis and metastasis.Experimental & molecular medicine · 2022Review
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- CDK phosphorylation ofThe EMBO journal · 2019Article
- HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- High-quality frozen extracts ofMolecular biology of the cell · 2017Article
- Metaphase Spindle Assembly.Biology · 2017Review
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- A unique insertion in STARD9's motor domain regulates its stability.Molecular biology of the cell · 2015Article
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- Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly.Developmental cell · 2013Article
- Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Acentrosomal spindle assembly and chromosome segregation during oocyte meiosis.Trends in cell biology · 2012Review
- The microtubule cytoskeleton is required for a G2 cell cycle delay in cancer cells lacking stathmin and p53.Cytoskeleton (Hoboken, N.J.) · 2012Article
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- Nup98 regulates bipolar spindle assembly through association with microtubules and opposition of MCAK.Molecular biology of the cell · 2011Article
- A computational model predicts Xenopus meiotic spindle organization.The Journal of cell biology · 2010Article
- The substrates of Plk1, beyond the functions in mitosis.Protein & cell · 2010Review
- Differential proteomic analysis of a highly metastatic variant of human breast cancer cells using two-dimensional differential gel electrophoresis.Journal of cancer research and clinical oncology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oncoprotein 18 (Op18) is a microtubule-destabilizing protein that is negatively regulated by phosphorylation. To evaluate the role of the three Op18 phosphorylation sites in Xenopus (Ser 16, 25, and 39), we added wild-type Op18, a nonphosphorylatable triple Ser to Ala mutant (Op18-AAA), and to mimic phosphorylation, a triple Ser to Glu mutant (Op18-EEE) to egg extracts and monitored spindle assembly. Op18-AAA dramatically decreased microtubule length and density, while Op18-EEE did not significantly affect spindle microtubules. Affinity chromatography with these proteins revealed that the microtubule-destabilizing activity correlated with the ability of Op18 to bind tubulin. Since hyperphosphorylation of Op18 is observed upon addition of mitotic chromatin to extracts, we reasoned that chromatin-associated proteins might play a role in Op18 regulation. We have performed a preliminary characterization of the chromatin proteins recruited to DNA beads, and identified the Xenopus polo-like kinase Plx1 as a chromatin-associated kinase that regulates Op18 phosphorylation. Depletion of Plx1 inhibits chromatin-induced Op18 hyperphosphorylation and spindle assembly in extracts. Therefore, Plx1 may promote microtubule stabilization and spindle assembly by inhibiting Op18.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.