ArticleThe EMBO journal2001
An in vitro evolved precursor tRNA with aminoacylation activity.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 144 citations in OpenAlex.
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- A reprogrammed genetic code consisting of 32 distinct amino acids.Nucleic acids research · 2026Article
- Functional Analysis of Histidine-Dependent Self-Cleaving RNAs Obtained by In Vitro Selection.Chembiochem : a European journal of chemical biology · 2025Article
- Promoting ribosomal incorporation of backbone-modifying nonproteinogenic amino acids into nascent peptides by ATP-binding cassette family-F proteins and EF-P.Nucleic acids research · 2025Article
- Discovering covalent cyclic peptide inhibitors of peptidyl arginine deiminase 4 (PADI4) using mRNA-display with a genetically encoded electrophilic warhead.Communications chemistry · 2024Article
- Reprogramming the genetic code with flexizymes.Nature reviews. Chemistry · 2024Review
- Prebiotic chiral transfer from self-aminoacylating ribozymes may favor either handedness.Nature communications · 2024Article
- Fine-tuning the tRNA anticodon arm for multiple/consecutive incorporations of β-amino acids and analogs.Nucleic acids research · 2024Article
- An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA in situ of a custom-made translation system for incorporation.Nucleic acids research · 2024Article
- Biocompatible strategies for peptide macrocyclisation.Chemical science · 2024Review
- A comprehensive analysis of translational misdecoding pattern and its implication on genetic code evolution.Nucleic acids research · 2023Article
- Translation initiation with exotic amino acids using EF-P-responsive artificial initiator tRNA.Nucleic acids research · 2023Article
- Identification of photocrosslinking peptide ligands by mRNA display.Communications chemistry · 2023Article
- Drop-off-reinitiation at the amino termini of nascent peptides and its regulation by IF3, EF-G, and RRF.RNA (New York, N.Y.) · 2023Article
- Ribosomal incorporation of negatively charged d-α- andPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Cell-free Biosynthesis of Peptidomimetics.Biotechnology and bioprocess engineering : BBE · 2023Review
- Emergent properties as by-products of prebiotic evolution of aminoacylation ribozymes.Nature communications · 2022Article
- Expansion of the Genetic Code Through the Use of Modified Bacterial Ribosomes.Journal of molecular biology · 2022Review
- Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P.Nucleic acids research · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
A set of catalysts for aminoacyl-tRNA synthesis is an essential component for translation. The RNA world hypothesis postulates that RNA catalysts could have played this role. Here we show an in vitro evolved precursor tRNA consisting of two domains, a catalytic 5'-leader sequence and an aminoacyl-acceptor tRNA. The 5'-leader sequence domain selectively self-charges phenylalanine on the 3'-terminus of the tRNA domain. This cis-acting ribozyme is susceptible to RNase P RNA, generating the corresponding 5'-leader segment and the mature tRNA. Moreover, the 5'-leader segment is able to aminoacylate the mature tRNA in trans. Mutational studies have revealed that C(74) and C(75) at the tRNA aminoacyl-acceptor end form base pairs with G71 and G70 of the trans-acting ribozyme. Such Watson-Crick base pairing with tRNA has been observed in RNase P RNA and 23S rRNA, suggesting that all three ribozymes use a similar mechanism for the recognition of the aminoacyl-acceptor end. Our demonstrations indicate that catalytic precursor tRNAs could have provided the foundations for the genetic coding system in the proto-translation system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.