ArticleThe EMBO journal2001
GPI anchoring leads to sphingolipid-dependent retention of endocytosed proteins in the recycling endosomal compartment.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 151 citations in OpenAlex.
- Fundamental Neurochemistry Review: Sphingolipids and Ceramides in Brain Development.Journal of neurochemistry · 2025Review
- Autologous Paracrine Prostasin-Matriptase Serine Protease Interaction in Lymphoid Cancer Cells.Cells · 2025Article
- Folate-mediated Transport of Nanoparticles across the Placenta.Pharmaceutical nanotechnology · 2024Article
- A novel GPI-anchored dominant-negative TGF-β receptor II renders T cells unresponsive to TGF-β signaling.Molecular therapy oncolytics · 2023Article
- Exosome-Mediated Activation of the Prostasin-Matriptase Serine Protease Cascade in B Lymphoma Cells.Cancers · 2023Article
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- Prostasin regulates PD-L1 expression in human lung cancer cells.Bioscience reports · 2021Article
- RAS Nanoclusters Selectively Sort Distinct Lipid Headgroups and Acyl Chains.Frontiers in molecular biosciences · 2021Review
- Article
- Improving cellular uptake of therapeutic entities through interaction with components of cell membrane.Drug delivery · 2019Review
- Possible Role of Phosphatidylcholine and Sphingomyelin on Fumonisin B1-mediated Toxicity.Food safety (Tokyo, Japan) · 2017Review
- Cholesterol dependence of collagen and echovirus 1 trafficking along the novel α2β1 integrin internalization pathway.PloS one · 2013Article
- Epigenetic modulation of the biophysical properties of drug-resistant cell lipids to restore drug transport and endocytic functions.Molecular pharmaceutics · 2012Article
- Glycosylphosphatidylinositol anchor analogues sequester cholesterol and reduce prion formation.The Journal of biological chemistry · 2010Article
- Endocytosis of glycosylphosphatidylinositol-anchored proteins.Journal of biomedical science · 2009Review
- HIV-1 Nef promotes endocytosis of cell surface MHC class II molecules via a constitutive pathway.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Identification of an intracellular site of prion conversion.PLoS pathogens · 2009Article
- Fyn tyrosine kinase regulates the surface expression of glycosylphosphatidylinositol-linked ephrin via the modulation of sphingomyelin metabolism.The Journal of biological chemistry · 2009Article
- Regulation of group I metabotropic glutamate receptor trafficking and signaling by the caveolar/lipid raft pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glycosylphosphatidylinositol (GPI) anchoring is important for the function of several proteins in the context of their membrane trafficking pathways. We have shown previously that endocytosed GPI-anchored proteins (GPI-APs) are recycled to the plasma membrane three times more slowly than other membrane components. Recently, we found that GPI-APs are delivered to endocytic organelles, devoid of markers of the clathrin-mediated pathway, prior to their delivery to a common recycling endosomal compartment (REC). Here we show that the rate-limiting step in the recycling of GPI-APs is their slow exit from the REC; replacement of the GPI anchor with a transmembrane protein sequence abolishes retention in this compartment. Depletion of endogenous sphingolipid levels using sphingolipid synthesis inhibitors or in a sphingolipid-synthesis mutant cell line specifically enhances the rate of endocytic recycling of GPI-APs to that of other membrane components. We have shown previously that endocytic retention of GPI-APs is also relieved by cholesterol depletion. These findings strongly suggest that functional retention of GPI-APs in the REC occurs via their association with sphingolipid and cholesterol-enriched sorting platforms or 'rafts'.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.