ArticleThe Plant cell2001
Functional characterization of a maize purine transporter by expression in Aspergillus nidulans.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 73 citations in OpenAlex.
- Transcriptomic and physiological effects of superabsorbent polymer seed coating on maize under drought stress.Frontiers in plant science · 2026Article
- Nucleobase-ascorbate transporter OsNAT9 regulates seed vigor and drought tolerance by modulating ascorbic acid homeostasis in rice.The Plant journal : for cell and molecular biology · 2025Article
- Purine metabolism in plant pathogenic fungi.Frontiers in microbiology · 2024Review
- An miR156-regulated nucleobase-ascorbate transporter 2 confers cadmium tolerance via enhanced anti-oxidative capacity in barley.Journal of advanced research · 2023Article
- Molecular Regulation and Evolution of Redox Homeostasis in Photosynthetic Machinery.Antioxidants (Basel, Switzerland) · 2022Review
- Exogenous application of xanthine and uric acid and nucleobase-ascorbate transporter MdNAT7 expression regulate salinity tolerance in apple.BMC plant biology · 2021Article
- Specific Residues in a Purine Transporter Are Critical for Dimerization, ER Exit, and Function.Genetics · 2019Article
- The solute transport and binding profile of a novel nucleobase cation symporter 2 from the honeybee pathogenFEBS open bio · 2018Article
- Evolution of substrate specificity in the Nucleobase-Ascorbate Transporter (NAT) protein family.Microbial cell (Graz, Austria) · 2018Article
- Comparative Genomics, Whole-Genome Re-sequencing and Expression Profile Analysis of Nucleobase:Cation Symporter 2 (Frontiers in plant science · 2018Article
- Uric acid in plants and microorganisms: Biological applications and genetics - A review.Journal of advanced research · 2017Review
- Dimeric structure of the uracil:proton symporter UraA provides mechanistic insights into the SLC4/23/26 transporters.Cell research · 2017Article
- Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato.Plant molecular biology · 2016Article
- The solute specificity profiles of nucleobase cation symporter 1 (NCS1) from Zea mays and Setaria viridis illustrate functional flexibility.Protoplasma · 2016Article
- Patterns of evolutionary conservation of ascorbic acid-related genes following whole-genome triplication in Brassica rapa.Genome biology and evolution · 2014Article
- Nucleobase and nucleoside transport and integration into plant metabolism.Frontiers in plant science · 2014Review
- Insights to the evolution of Nucleobase-Ascorbate Transporters (NAT/NCS2 family) from the Cys-scanning analysis of xanthine permease XanQ.International journal of biochemistry and molecular biology · 2012Article
- Identification of candidate genes associated with senescence in durum wheat (Triticum turgidum subsp. durum) using cDNA-AFLP.Molecular biology reports · 2011Article
- Identification and functional characterization of the first nucleobase transporter in mammals: implication in the species difference in the intestinal absorption mechanism of nucleobases and their analogs between higher primates and other mammals.The Journal of biological chemistry · 2010Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the function of Leaf Permease1 (LPE1), a protein that is necessary for proper chloroplast development in maize, by functional expression in the filamentous fungus Aspergillus nidulans. The choice of this ascomycete was dictated by the similarity of its endogenous purine transporters to LPE1 and by particular genetic and physiological features of purine transport and metabolism in A. nidulans. When Lpe1 was expressed in a purine transport-deficient A. nidulans strain, the capacity for uric acid and xanthine transport was acquired. This capacity was directly dependent on Lpe1 copy number and expression level. Interestingly, overexpression of LPE1 from >10 gene copies resulted in transformants with pleiotropically reduced growth rates on various nitrogen sources and the absolute inability to transport purines. Kinetic analysis established that LPE1 is a high-affinity (K(m) = 30 +/- 2.5 microM), high-capacity transporter specific for the oxidized purines xanthine and uric acid. Competition studies showed that high concentrations of ascorbic acid (>30 mM) competitively inhibit LPE1-mediated purine transport. This work defines the biochemical function of LPE1, a plant representative of a large and ubiquitous transporter family. In addition, A. nidulans is introduced as a novel model system for the cloning and/or functional characterization of transporter genes.
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