ArticleMolecular and cellular biology2001
runt homology domain transcription factors (Runx, Cbfa, and AML) mediate repression of the bone sialoprotein promoter: evidence for promoter context-dependent activity of Cbfa proteins.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 177 citations in OpenAlex.
- The ATF3-OPG Axis Contributes to Bone Formation by Regulating the Differentiation of Osteoclasts, Osteoblasts, and Adipocytes.International journal of molecular sciences · 2022Article
- Why SNP rs3755955 is associated with human bone mineral density? A molecular and cellular study in bone cells.Molecular and cellular biochemistry · 2022Article
- Adiponectin inhibits lipoplysaccharide-induced inflammation and promotes osteogenesis in hPDLCs.Bioscience reports · 2021Article
- Effect of Qing'e Decoction on Leptin/Leptin Receptor and Bone Metabolism in Naturally Aging Rats.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- RUNX family: Oncogenes or tumor suppressors (Review).Oncology reports · 2019Review
- Peroxiredoxin II negatively regulates BMP2-induced osteoblast differentiation and bone formation via PP2A Cα-mediated Smad1/5/9 dephosphorylation.Experimental & molecular medicine · 2019Article
- Overexpression of Dlx2 enhances osteogenic differentiation of BMSCs and MC3T3-E1 cells via direct upregulation of Osteocalcin and Alp.International journal of oral science · 2019Article
- Contrasting patterns of RUNX2 repeat variations are associated with palate shape in phyllostomid bats and New World primates.Scientific reports · 2018Article
- Kisspeptin-10 (KP-10) stimulates osteoblast differentiation through GPR54-mediated regulation of BMP2 expression and activation.Scientific reports · 2018Article
- COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring.Scientific reports · 2017Article
- Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic.Experimental & molecular medicine · 2017Article
- Article
- Inhibition of Runx2 signaling by TNF-α in ST2 murine bone marrow stromal cells undergoing osteogenic differentiation.In vitro cellular & developmental biology. Animal · 2016Article
- Vibrational stimulation induces osteoblast differentiation and the upregulation of osteogenic gene expression in vitro.Cytotechnology · 2016Article
- Transcriptional Auto-Regulation of RUNX1 P1 Promoter.PloS one · 2016Article
- Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?Experimental and therapeutic medicine · 2015Article
- Genomic occupancy of Runx2 with global expression profiling identifies a novel dimension to control of osteoblastogenesis.Genome biology · 2014Article
- BMP-2 differentially modulates FGF-2 isoform effects in osteoblasts from newborn transgenic mice.Endocrinology · 2013Article
- Effects of titanium surface anodization with CaP incorporation on human osteoblastic response.Materials science & engineering. C, Materials for biological applications · 2013Article
- BMP2 protein regulates osteocalcin expression via Runx2-mediated Atf6 gene transcription.The Journal of biological chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Expression of the bone sialoprotein (BSP) gene, a marker of bone formation, is largely restricted to cells in mineralized tissues. Recent studies have shown that the Cbfa1 (also known as Runx2, AML-3, and PEBP2alphaA) transcription factor supports commitment and differentiation of progenitor cells to hypertrophic chondrocytes and osteoblasts. This study addresses the functional involvement of Cbfa sites in expression of the Gallus BSP gene. Gel mobility shift analyses with nuclear extracts from ROS 17/2.8 osteoblastic cells revealed that multiple Cbfa consensus sequences are functional Cbfa DNA binding sites. Responsiveness of the 1.2-kb Gallus BSP promoter to Cbfa factors Cbfa1, Cbfa2, and Cbfa3 was assayed in osseous and nonosseous cells. Each of the Cbfa factors mediated repression of the wild-type BSP promoter, in contrast to their well known activation of various hematopoietic and skeletal phenotypic genes. Suppression of BSP by Cbfa factors was not observed in BSP promoters in which Cbfa sites were deleted or mutated. Expression of the endogenous BSP gene in Gallus osteoblasts was similarly downregulated by forced expression of Cbfa factors. Our data indicate that Cbfa repression of the BSP promoter does not involve the transducin-like enhancer (TLE) proteins. Neither coexpression of TLE1 or TLE2 nor the absence of the TLE interaction motif of Cbfa1 (amino acids 501 to 513) influenced repressor activity. However, removal of the C terminus of Cbfa1 (amino acids 362 to 513) relieved suppression of the BSP promoter. Our results, together with the evolutionary conservation of the seven Cbfa sites in the Gallus and human BSP promoters, suggest that suppressor activity by Cbfa is of significant physiologic consequence and may contribute to spatiotemporal expression of BSP during bone development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.