ArticleMolecular and cellular biology2001
Novel fluorescence-based screen to identify small synthetic internal ribosome entry site elements.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Review
- Mechanistic insights into Sindbis virus infection: noncapped genomic RNAs enhance the translation of capped genomic RNAs to promote viral infectivity.Nucleic acids research · 2025Article
- Therapeutic homology-independent targeted integration in retina and liver.Nature communications · 2022Article
- A Platform Technology for Monitoring the Unfolded Protein Response.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Targeting IRES-dependent translation as a novel approach for treating Duchenne muscular dystrophy.RNA biology · 2021Review
- A gene signal amplifier platform for monitoring the unfolded protein response.Nature chemical biology · 2020Article
- Intracellular Imaging with Genetically Encoded RNA-based Molecular Sensors.Nanomaterials (Basel, Switzerland) · 2019Review
- Synthetic biology: advancing the design of diverse genetic systems.Annual review of chemical and biomolecular engineering · 2013Review
- A biobrick library for cloning custom eukaryotic plasmids.PloS one · 2011Article
- Synthetic biology: tools to design, build, and optimize cellular processes.Journal of biomedicine & biotechnology · 2010Review
- Translational modulation of proteins expressed from bicistronic vectors.Molecular imaging · 2009Article
- The heat shock protein inhibitor Quercetin attenuates hepatitis C virus production.Hepatology (Baltimore, Md.) · 2009Article
- Zuotin, a DnaJ molecular chaperone, stimulates cap-independent translation in yeast.Biochemical and biophysical research communications · 2006Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- beta-Adrenoreceptors reactivate Kaposi's sarcoma-associated herpesvirus lytic replication via PKA-dependent control of viral RTA.Journal of virology · 2005Article
- A positive feedback vector for identification of nucleotide sequences that enhance translation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Systematic analysis of bicistronic reporter assay data.Nucleic acids research · 2004Article
- A peptide from autoantigen La blocks poliovirus and hepatitis C virus cap-independent translation and reveals a single tyrosine critical for La RNA binding and translation stimulation.Journal of virology · 2004Article
- Conserved nucleotides within the J domain of the encephalomyocarditis virus internal ribosome entry site are required for activity and for interaction with eIF4G.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We report here a novel fluorescent protein-based screen to identify small, synthetic internal ribosome entry site (IRES) elements in vivo. A library of bicistronic plasmids encoding the enhanced blue and green fluorescent proteins (EBFP and EGFP) separated by randomized 50-nucleotide-long sequences was amplified in bacteria and delivered into mammalian cells via protoplast fusion. Cells that received functional IRES elements were isolated using the EBFP and EGFP reporters and fluorescence-activated cell sorting, and several small IRES elements were identified. Two of these elements were subsequently shown to possess IRES activity comparable to that of a variant of the encephalomyocarditis virus IRES element in a context-independent manner both in vitro and in vivo, and these elements functioned in multiple cell types. Although no sequence or structural homology was apparent between the synthetic IRES elements and known viral and cellular IRES elements, the two synthetic IRES elements specifically blocked poliovirus (PV) IRES-mediated translation in vitro. Competitive protein-binding experiments suggested that these IRES elements compete with PV IRES-mediated translation by utilizing some of the same factors as the PV IRES to direct translation. The utility of this fluorescent protein-based screen in identifying IRES elements with improved activity as well as in probing the mechanism of IRES-mediated translation is discussed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.