ArticleMolecular and cellular biology2001
UvsW protein regulates bacteriophage T4 origin-dependent replication by unwinding R-loops.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- The bacteriophage T4 homologous recombination system: mechanism, applications, conservation, and environmental significance.EcoSal Plus · 2025Review
- MazF endoribonuclease promotes resolution of transcription-replication conflicts at ribosomal RNA genes in Escherichia coli.Nucleic acids research · 2025Article
- A real-time fluorescent gp32 probe-based assay for monitoring single-stranded DNA-dependent DNA processing enzymes.Biochemistry and biophysics reports · 2023Article
- rRNA operon multiplicity as a bacterial genome stability insurance policy.Nucleic acids research · 2022Article
- Modulation of RecFORQ- and RecA-Mediated Homologous Recombination in Escherichia coli by Isoforms of Translation Initiation Factor IF2.Journal of bacteriology · 2022Article
- Bacteriophage-Encoded DNA Polymerases-Beyond the Traditional View of Polymerase Activities.International journal of molecular sciences · 2022Review
- Topoisomerase I Essentiality, DnaA-Independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli.Journal of bacteriology · 2021Article
- Replisome bypass of transcription complexes and R-loops.Nucleic acids research · 2020Article
- Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli.Nucleic acids research · 2018Article
- Bacteriophage T5 gene D10 encodes a branch-migration protein.Scientific reports · 2016Article
- The RECG1 DNA Translocase Is a Key Factor in Recombination Surveillance, Repair, and Segregation of the Mitochondrial DNA in Arabidopsis.The Plant cell · 2015Article
- Ribonucleotides in bacterial DNA.Critical reviews in biochemistry and molecular biology · 2015Review
- Unreported intrinsic disorder in proteins: Building connections to the literature on IDPs.Intrinsically disordered proteins · 2014Article
- Interaction of T4 UvsW helicase and single-stranded DNA binding protein gp32 through its carboxy-terminal acidic tail.Journal of molecular biology · 2013Article
- Rho-dependent transcription termination is essential to prevent excessive genome-wide R-loops in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The genome and proteome of a Campylobacter coli bacteriophage vB_CcoM-IBB_35 reveal unusual features.Virology journal · 2012Article
- Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives.Virology journal · 2010Review
- Assembly and dynamics of the bacteriophage T4 homologous recombination machinery.Virology journal · 2010Review
- Analysis of the DNA translocation and unwinding activities of T4 phage helicases.Methods (San Diego, Calif.) · 2010Article
- Processive and unidirectional translocation of monomeric UvsW helicase on single-stranded DNA.Biochemistry · 2009Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The UvsW protein of bacteriophage T4 is involved in many aspects of phage DNA metabolism, including repair, recombination, and recombination-dependent replication. UvsW has also been implicated in the repression of origin-dependent replication at late times of infection, when UvsW is normally synthesized. Two well-characterized T4 origins, ori(uvsY) and ori(34), are believed to initiate replication through an R-loop mechanism. Here we provide both in vivo and in vitro evidence that UvsW is an RNA-DNA helicase that catalyzes the dissociation of RNA from origin R-loops. Two-dimensional gel analyses show that the replicative intermediates formed at ori(uvsY) persist longer in a uvsW mutant infection than in a wild-type infection. In addition, the inappropriate early expression of UvsW protein results in the loss of these replicative intermediates. Using a synthetic origin R-loop, we also demonstrate that purified UvsW functions as a helicase that efficiently dissociates RNA from R-loops. These and previous results from a number of studies provide strong evidence that UvsW is a molecular switch that allows T4 replication to progress from a mode that initiates from R-loops at origins to a mode that initiates from D-loops formed by recombination proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.