ArticleJournal of clinical microbiology2001
Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it, 181 citations in OpenAlex.
- A Systematic Review and Meta-Analysis on the Prevalence of Brucella melitensis in Sheep and Goat.Veterinary medicine and science · 2025Pooled it
- Establishment of a rapid Brucella detection method based on MCDA-CRISPR dual signal amplification system for reducing transfusion-transmitted diseases.Scientific reports · 2026Article
- Seroprevalence and molecular detection of brucellosis among Pakistani women with spontaneous abortion.Frontiers in public health · 2024Article
- Comparative evaluation of RBPT, I-ELISA, and CFT for the diagnosis of brucellosis and PCR detection of Brucella species from Ethiopian sheep, goats, and cattle sera.BMC microbiology · 2023Article
- Review
- Brucellosis: epidemiology, pathogenesis, diagnosis and treatment-a comprehensive review.Annals of medicine · 2023Review
- Brucellosis in the Brazilian Pantanal wetland: threat to animal production and wildlife conservation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Review
- The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources ofVeterinary sciences · 2022Article
- Increased serum piwi-interacting RNAs as a novel potential diagnostic tool for brucellosis.Frontiers in cellular and infection microbiology · 2022Article
- Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview.Pathogens (Basel, Switzerland) · 2021Review
- Circulating Brucella species in wild animals of the Serengeti ecosystem, Tanzania.One health outlook · 2021Article
- Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis.Archives of Razi Institute · 2021Article
- Serological and molecular investigation of human brucellosis in participants of Famenin brucellosis cohort study, Hamadan, Iran.Iranian journal of microbiology · 2021Article
- Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.Scientific reports · 2021Article
- Global metabolomic analysis of blood from mice infected with Brucella abortus.The Journal of veterinary medical science · 2021Article
- Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera.Veterinary world · 2021Article
- Seroprevalence of brucellosis in sheep and goats in the Arabian Gulf region.Veterinary world · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Acute Febrile Illness Caused byInternational journal of environmental research and public health · 2019Article
- Fever, bacterial zoonoses, and One Health in sub-Saharan Africa.Clinical medicine (London, England) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human brucellosis poses a significant public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the 31-kDa Brucella abortus antigenic protein gene sequence was developed and applied to whole-blood and serum samples from 31 brucellosis patients and 45 healthy individuals. All patients except one had detectable Brucella DNA in either whole blood or serum (combined sensitivity, 97%), but the assay sensitivity was higher with serum samples (94%) than with whole-blood samples (61%). The assay specificity was excellent (100%). A confirmatory PCR assay targeting another Brucella gene region (omp-2) was also developed but lacked sensitivity. Serum is the optimal specimen for the diagnosis of brucellosis by PCR, a choice that leads to assay simplification and shortens turnaround time.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.