ArticleJournal of clinical microbiology2001
High degree of interlaboratory reproducibility of human immunodeficiency virus type 1 protease and reverse transcriptase sequencing of plasma samples from heavily treated patients.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Prospective Evaluation of the Vela Diagnostics Next-Generation Sequencing Platform for HIV-1 Genotypic Resistance Testing.The Journal of molecular diagnostics : JMD · 2019Article
- Comparison of anJournal of clinical microbiology · 2018Article
- HIV Resistance Prediction to Reverse Transcriptase Inhibitors: Focus on Open Data.Molecules (Basel, Switzerland) · 2018Review
- More effective drugs lead to harder selective sweeps in the evolution of drug resistance in HIV-1.eLife · 2016Article
- A comparison of two measures of HIV diversity in multi-assay algorithms for HIV incidence estimation.PloS one · 2014Article
- Evaluation of a cost effective in-house method for HIV-1 drug resistance genotyping using plasma samples.PloS one · 2014Article
- Cooperative effects of drug-resistance mutations in the flap region of HIV-1 protease.ACS chemical biology · 2013Article
- Antiviral resistance and correlates of virologic failure in the first cohort of HIV-infected children gaining access to structured antiretroviral therapy in Lima, Peru: a cross-sectional analysis.BMC infectious diseases · 2013Article
- Persistence versus reversion of 3TC resistance in HIV-1 determine the rate of emergence of NVP resistance.Viruses · 2012Article
- Automating HIV drug resistance genotyping with RECall, a freely accessible sequence analysis tool.Journal of clinical microbiology · 2012Article
- Genetic diversity of recently acquired and prevalent HIV, hepatitis B virus, and hepatitis C virus infections in US blood donors.The Journal of infectious diseases · 2012Article
- The Brazilian network for HIV-1 genotyping external quality control assurance programme.Journal of the International AIDS Society · 2011Article
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- Minority variants associated with transmitted and acquired HIV-1 nonnucleoside reverse transcriptase inhibitor resistance: implications for the use of second-generation nonnucleoside reverse transcriptase inhibitors.Journal of acquired immune deficiency syndromes (1999) · 2009Article
- TREAT Asia Quality Assessment Scheme (TAQAS) to standardize the outcome of HIV genotypic resistance testing in a group of Asian laboratories.Journal of virological methods · 2009Article
- Minority human immunodeficiency virus type 1 variants in antiretroviral-naive persons with reverse transcriptase codon 215 revertant mutations.Journal of virology · 2008Article
- Consensus drug resistance mutations for epidemiological surveillance: basic principles and potential controversies.Antiviral therapy · 2008Review
- Mapping protease inhibitor resistance to human immunodeficiency virus type 1 sequence polymorphisms within patients.Journal of virology · 2007Article
- N88D facilitates the co-occurrence of D30N and L90M and the development of multidrug resistance in HIV type 1 protease following nelfinavir treatment failure.AIDS research and human retroviruses · 2006Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We assessed the reproducibility of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) and protease sequencing using cryopreserved plasma aliquots obtained from 46 heavily treated HIV-1-infected individuals in two laboratories using dideoxynucleotide sequencing. The rates of complete sequence concordance between the two laboratories were 99.1% for the protease sequence and 99.0% for the RT sequence. Approximately 90% of the discordances were partial, defined as one laboratory detecting a mixture and the second laboratory detecting only one of the mixture's components. Only 0.1% of the nucleotides were completely discordant between the two laboratories, and these were significantly more likely to occur in plasma samples with lower plasma HIV-1 RNA levels. Nucleotide mixtures were detected at approximately 1% of the nucleotide positions, and in every case in which one laboratory detected a mixture, the second laboratory either detected the same mixture or detected one of the mixture's components. The high rate of concordance in detecting mixtures and the fact that most discordances between the two laboratories were partial suggest that most discordances were caused by variation in sampling of the HIV-1 quasispecies by PCR rather than by technical errors in the sequencing process itself.
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