ArticleJournal of clinical microbiology2001
Chlamydial serology: comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 90 citations in OpenAlex.
- Proteostasis, Assisted Reproductive Technologies, and Neurodevelopmental Differences: An Integrative Perspective.Proteomes · 2026Review
- Chlamydia trachomatis Seroassays Used in Epidemiologic Research: A Narrative Review and Practical Considerations.The Journal of infectious diseases · 2024Review
- The serodiagnositic value ofFrontiers in public health · 2024Article
- Peptide ELISA and FRET-qPCR Identified a Significantly Higher Prevalence ofPathogens (Basel, Switzerland) · 2020Article
- Multi-peptide ELISAs overcome cross-reactivity and inadequate sensitivity of conventional Chlamydia pneumoniae serology.Scientific reports · 2019Article
- Article
- Performance of Chlamydia trachomatis OmcB Enzyme-Linked Immunosorbent Assay in Serodiagnosis of Chlamydia trachomatis Infection in Women.Journal of clinical microbiology · 2018Article
- Comprehensive Molecular Serology of HumanmSphere · 2018Article
- Discovery of Human-Specific ImmunodominantmSphere · 2018Article
- Article
- Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression.Scientific reports · 2018Article
- A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera.Scientific reports · 2018Article
- Molecular and serological dynamics ofPeerJ · 2018Article
- The potential role of infectious agents and pelvic inflammatory disease in ovarian carcinogenesis.Infectious agents and cancer · 2017Review
- Defining species-specific immunodominant B cell epitopes for molecular serology of Chlamydia species.Clinical and vaccine immunology : CVI · 2015Article
- CT694 and pgp3 as serological tools for monitoring trachoma programs.PLoS neglected tropical diseases · 2012Article
- Serum lipoproteins attenuate macrophage activation and Toll-Like Receptor stimulation by bacterial lipoproteins.BMC immunology · 2010Article
- Comparison of five commercial serological tests for the detection of anti-Chlamydia trachomatis antibodies.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
- Identification of immunodominant antigens of Chlamydia trachomatis using proteome microarrays.Vaccine · 2010Article
- Chlamydial infection: the link with ocular adnexal lymphomas.Nature reviews. Clinical oncology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve the reliability of the serodiagnosis of Chlamydia trachomatis infections, an immunoblot analysis, a microimmunofluorescence titration, and different immunoassays using synthetic peptides derived from species-specific epitopes in variable domain IV of the major outer membrane protein or recombinant antigens (heat shock protein 70 [hsp70], hsp60, hsp10, polypeptide encoded by open reading frame 3 of the plasmid [pgp3], macrophage infectivity potentiator, and a fragment of the total lipopolysaccharide) were evaluated. Because cross-reactions between chlamydial species have been reported, the microimmunofluorescence tests were also performed with Chlamydia pneumoniae and Chlamydia psittaci used as antigens, and C. pneumoniae-specific antibodies were also determined by immunoassays. Since the presence of antimicrobial antibodies must be interpreted in light of their prevalence in the general population, responses obtained with serum samples from patients with well-defined infection (i.e., with positive urethral or endocervical C. trachomatis DNA amplification) were compared to those obtained with samples from healthy blood donors. The best sensitivity (86%) with a specificity of 81% was obtained for immunoblotting results, when the number of individuals with > or =10 immunoglobulin G (IgG) and/or > or =2 IgM responses to the different C. trachomatis antigens was considered. A 13-kDa antigen was recognized by most of the samples (86% for IgG) from patients with acute urogenital infection but rarely (3%) by those from healthy blood donors (P < 0.0001). The sensitivity and specificity results obtained for serum antibodies to peptides or recombinant antigens were slightly lower than those results obtained for the number of responses to whole C. trachomatis antigens, which were 76 and 77%, respectively, when IgG responses to both recombinant hsp60 and pgp3 were considered.
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