ArticleJournal of clinical microbiology2001
Detection of Mycobacterium bovis in bovine clinical specimens using real-time fluorescence and fluorescence resonance energy transfer probe rapid-cycle PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- The Risk of False-Positive Serological Results for Paratuberculosis inPathogens (Basel, Switzerland) · 2021Article
- Direct PCR on Tissue Samples To Detect Mycobacterium tuberculosis Complex: an Alternative to the Bacteriological Culture.Journal of clinical microbiology · 2021Article
- Bayesian Assessment of the Accuracy of a PCR-Based Rapid Diagnostic Test for Bovine Tuberculosis in Swine.Frontiers in veterinary science · 2019Article
- Validation of a Real-Time PCR for the Detection ofFrontiers in veterinary science · 2019Article
- Prevalence of bovine tuberculosis in slaughtered cattle identified by nested-PCR in abattoirs from two dairy areas of Ecuador.Tropical animal health and production · 2014Article
- Standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover Mycobacterium tuberculosis complex members.Frontiers in molecular biosciences · 2014Review
- Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Use of cell-free circulating schistosome DNA in serum, urine, semen, and saliva to monitor a case of refractory imported schistosomiasis hematobia.Journal of clinical microbiology · 2013Article
- Enhanced detection of tuberculous mycobacteria in animal tissues using a semi-nested probe-based real-time PCR.PloS one · 2013Article
- Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis.Veterinary medicine international · 2012Article
- Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.The Journal of molecular diagnostics : JMD · 2009Article
- Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR.BMC veterinary research · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk.Applied and environmental microbiology · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
- Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.Journal of clinical microbiology · 2003Article
- Simultaneous identification of Mycobacterium genus and Mycobacterium tuberculosis complex in clinical samples by 5'-exonuclease fluorogenic PCR.Journal of clinical microbiology · 2002Article
- Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.Journal of clinical microbiology · 2002Article
- Rapid-cycle PCR and fluorimetry for detection of mycobacteria.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid sequence capture extraction was coupled with LightCycler PCR amplification and product detection using real-time fluorescence for rapid, definitive detection of Mycobacterium bovis in lymph node specimens from 38 cattle with bovine tuberculosis lesions. PCR amplification of sequence-captured DNA using both a conventional heating block thermocycler and a LightCycler thermocycler was compared with culture and histopathological analyses. Conventional PCR enabled detection of 26 of 28 culture-positive specimens (93%) in approximately 9 h, and the LightCycler PCR detected 20 of 28 culture-positive specimens (71%) in only 30 min. Specific confirmation of Mycobacterium tuberculosis complex DNA was achieved by LightCycler PCR amplification using Syb Green 1 and an M. tuberculosis complex-specific Cy5-labeled fluorescence resonance energy transfer probe. The system described here enabled rapid and specific laboratory confirmation of bovine tuberculosis, and this is the first report of the detection of M. bovis in tissues using LightCycler PCR. The fluorescence technology used in the study has potential to allow development of a high-throughput molecular diagnostic test for bovine tuberculosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.