ArticleGenome research2001
Homogeneous assays for single-nucleotide polymorphism typing using AlphaScreen.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- High-throughput homogenous assay for the direct detection of Listeria monocytogenes DNA.Scientific reports · 2024Article
- Flow-Through Electrochemical Biosensor for the Detection ofSensors (Basel, Switzerland) · 2021Article
- Quantitation of estradiol by competitive light-initiated chemiluminescent assay using estriol as competitive antigen.Journal of clinical laboratory analysis · 2020Article
- Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses.Applied biochemistry and biotechnology · 2014Review
- Aptamers recognizing glycosylated hemagglutinin expressed on the surface of vaccinia virus-infected cells.Analytical chemistry · 2010Article
- Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.Journal of proteome research · 2010Article
- The use of AlphaScreen technology in HTS: current status.Current chemical genomics · 2008Article
- Quantification of low concentrations of DNA using single molecule detection and velocity measurement in a microchannel.Journal of fluorescence · 2007Article
- Phosphoprotein analysis: from proteins to proteomes.Proteome science · 2006Article
- Gametic phase estimation over large genomic regions using an adaptive window approach.Human genomics · 2003Article
- Genotyping single nucleotide polymorphisms directly from genomic DNA by invasive cleavage reaction on microspheres.Nucleic acids research · 2003Article
- Progress in high throughput SNP genotyping methods.The pharmacogenomics journal · 2002Review
- Bayesian haplotype inference for multiple linked single-nucleotide polymorphisms.American journal of human genetics · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AlphaScreen technology allows the development of high-throughput homogeneous proximity assays. In these assays, signal is generated when 680 nm laser light irradiates a donor bead in close proximity to an acceptor bead. For the detection of nucleic acids, donor and acceptor beads are brought into proximity by two bridging probes that hybridize simultaneously to a common target and to the generic oligonucleotides attached covalently to the beads. This method allows the detection of as little as 10 amole of a single-stranded DNA target. The combination of AlphaScreen with allele-specific amplification (ASA) and allele-specific hybridization (ASH) has allowed the development of two homogenous single-nucleotide polymorphism (SNP) genotyping platforms. Both types of assay are very robust, routinely giving accurate genotyping results with < 2 ng of genomic DNA per genotype. An AlphaScreen validation study was performed for 12 SNPs by using ASA assays and seven SNPs by using ASH assays. More than 580 samples were genotyped with accuracy >99%. The two assays are remarkably simple, requiring no post-PCR manipulations. Genotyping has been performed successfully in 96- and 384-well formats with volumes as small as 2 microL, allowing a considerable reduction in the amount of reagents and genomic DNA necessary for genotyping. These results show that the AlphaScreen technology can be successfully adapted to high-throughput genotyping.
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