ArticleApplied and environmental microbiology2001
Determination of DNA content of aquatic bacteria by flow cytometry.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
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- Anti-bacterial and anti-biofilm activities of arachidonic acid against the cariogenic bacteriumFrontiers in microbiology · 2024Article
- The unique chemical and microbiological signatures of an array of bottled drinking water.Frontiers in microbiology · 2024Article
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- Characterization of archaeal and bacterial communities thriving in methane-seeping on-land mud volcanoes, Niigata, Japan.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Historical contingencies and phage induction diversify bacterioplankton communities at the microscale.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- DAPI staining and DNA content estimation of nuclei in uncultivable microbial eukaryotes (Arcellinida and Ciliates).European journal of protistology · 2021Article
- Anandamide alters the membrane properties, halts the cell division and prevents drug efflux in multidrug resistant Staphylococcus aureus.Scientific reports · 2021Article
- Evaluation of DNA extraction yield from a chlorinated drinking water distribution system.PloS one · 2021Article
- Article
- Auxotrophic interactions: a stabilizing attribute of aquatic microbial communities?FEMS microbiology ecology · 2020Article
- Regulation of Low and High Nucleic Acid Fluorescent Heterotrophic Prokaryote Subpopulations and Links to Viral-Induced Mortality Within Natural Prokaryote-Virus Communities.Microbial ecology · 2020Article
- Structure and biogenesis of ribonucleoprotein bodies in epidermal cells of Caragana arborescens L.Protoplasma · 2018Article
- Low-Temperature Sulfidic-Ice Microbial Communities, Borup Fiord Pass, Canadian High Arctic.Frontiers in microbiology · 2018Article
- Energy Landscapes in Hydrothermal Chimneys Shape Distributions of Primary Producers.Frontiers in microbiology · 2018Article
- Wavelength-normalized spectroscopic analysis ofBiomedical optics express · 2016Article
- Article
- Geomicrobiological Features of Ferruginous Sediments from Lake Towuti, Indonesia.Frontiers in microbiology · 2016Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distribution of DNA among bacterioplankton and bacterial isolates was determined by flow cytometry of DAPI (4',6'-diamidino-2-phenylindole)-stained organisms. Conditions were optimized to minimize error from nonspecific staining, AT bias, DNA packing, changes in ionic strength, and differences in cell permeability. The sensitivity was sufficient to characterize the small 1- to 2-Mb-genome organisms in freshwater and seawater, as well as low-DNA cells ("dims"). The dims could be formed from laboratory cultivars; their apparent DNA content was 0.1 Mb and similar to that of many particles in seawater. Preservation with formaldehyde stabilized samples until analysis. Further permeabilization with Triton X-100 facilitated the penetration of stain into stain-resistant lithotrophs. The amount of DNA per cell determined by flow cytometry agreed with mean values obtained from spectrophotometric analyses of cultures. Correction for the DNA AT bias of the stain was made for bacterial isolates with known G+C contents. The number of chromosome copies per cell was determined with pure cultures, which allowed growth rate analyses based on cell cycle theory. The chromosome ratio was empirically related to the rate of growth, and the rate of growth was related to nutrient concentration through specific affinity theory to obtain a probe for nutrient kinetics. The chromosome size of a Marinobacter arcticus isolate was determined to be 3.0 Mb by this method. In a typical seawater sample the distribution of bacterial DNA revealed two major populations based on DNA content that were not necessarily similar to populations determined by using other stains or protocols. A mean value of 2.5 fg of DNA cell(-1) was obtained for a typical seawater sample, and 90% of the population contained more than 1.1 fg of DNA cell(-1).
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