ArticleApplied and environmental microbiology2001
Investigating microbial (micro)colony heterogeneity by vibrational spectroscopy.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Laboratory strategies for the identification of Burkholderia species: From classical phenotyping to advanced genomic and proteomic approaches.Antonie van Leeuwenhoek · 2026Review
- Fourier transform infrared spectroscopy enables rapid strain typing and cluster analysis ofFrontiers in microbiology · 2026Article
- The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia).World journal of microbiology & biotechnology · 2025Article
- The impact of phenotypic heterogeneity on fungal pathogenicity and drug resistance.FEMS microbiology reviews · 2025Review
- Lighting the Path: Raman Spectroscopy's Journey Through the Microbial Maze.Molecules (Basel, Switzerland) · 2024Article
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Raman Spectroscopy-A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings.Frontiers in cellular and infection microbiology · 2022Review
- Article
- Biochemical characterization of pathogenic bacterial species using Raman spectroscopy and discrimination model based on selected spectral features.Lasers in medical science · 2021Article
- Fourier Transform Infrared Spectroscopy: An Innovative Method for the Diagnosis of Ovarian Cancer.Cancer management and research · 2021Review
- Toxicity mechanism of CuJournal of biological physics · 2020Article
- Evaluation of the impact of buffered peptone water composition on the discrimination between Salmonella enterica and Escherichia coli by Raman spectroscopy.Analytical and bioanalytical chemistry · 2020Article
- The FT-IR and Raman Spectroscopies as Tools for Biofilm Characterization Created by Cariogenic Streptococci.International journal of molecular sciences · 2020Article
- Food Microstructure and Fat Content Affect Growth Morphology, Growth Kinetics, and Preferred Phase for Cell Growth ofApplied and environmental microbiology · 2019Article
- Standardization of complex biologically derived spectrochemical datasets.Nature protocols · 2019Article
- Comparative responses of diazotrophic abundance and community structure to the chemical composition of paddy soil.Environmental science and pollution research international · 2018Article
- Rapid identification of staphylococci by Raman spectroscopy.Scientific reports · 2017Article
- Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16.Sensors (Basel, Switzerland) · 2016Article
- Influence of Culture Media on Microbial Fingerprints Using Raman Spectroscopy.Sensors (Basel, Switzerland) · 2015Article
- Bacterial Colonies in Solid Media and Foods: A Review on Their Growth and Interactions with the Micro-Environment.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fourier transform infrared and Raman microspectroscopy are currently being developed as new methods for the rapid identification of clinically relevant microorganisms. These methods involve measuring spectra from microcolonies which have been cultured for as little as 6 h, followed by the nonsubjective identification of microorganisms through the use of multivariate statistical analyses. To examine the biological heterogeneity of microorganism growth which is reflected in the spectra, measurements were acquired from various positions within (micro)colonies cultured for 6, 12, and 24 h. The studies reveal that there is little spectral variance in 6-h microcolonies. In contrast, the 12- and 24-h cultures exhibited a significant amount of heterogeneity. Hierarchical cluster analysis of the spectra from the various positions and depths reveals the presence of different layers in the colonies. Further analysis indicates that spectra acquired from the surface of the colonies exhibit higher levels of glycogen than do the deeper layers of the colony. Additionally, the spectra from the deeper layers present with higher RNA levels than the surface layers. Therefore, the 6-h colonies with their limited heterogeneity are more suitable for inclusion in a spectral database to be used for classification purposes. These results also demonstrate that vibrational spectroscopic techniques can be useful tools for studying the nature of colony development and biofilm formation.
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Registered trials
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