ArticleProceedings of the National Academy of Sciences of the United States of America2001
Identification of a sequence element directing a protein to nuclear speckles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Phase Separation of SF3B1 Serves as a Critical Post-Transcriptional Regulator During Early Mouse Embryogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Igh nuclear speckle association promotes V(D)J recombination.Nature communications · 2026Article
- CDK7-CDK11 axis in spliceosome regulation and pre-mRNA splicing.Nucleic acids research · 2025Article
- A common structural mechanism for RNA recognition by the SF3B complex in mRNA splicing and export.Nucleic acids research · 2025Article
- Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing.Nature communications · 2022Article
- Early Diagnosis of Hepatocellular Carcinoma Using Machine Learning Method.Frontiers in bioengineering and biotechnology · 2020Article
- Detailed prediction of protein sub-nuclear localization.BMC bioinformatics · 2019Article
- Identification of anti-SF3B1 autoantibody as a diagnostic marker in patients with hepatocellular carcinoma.Journal of translational medicine · 2018Article
- C3G dynamically associates with nuclear speckles and regulates mRNA splicing.Molecular biology of the cell · 2018Article
- Principles of protein targeting to the nucleolus.Nucleus (Austin, Tex.) · 2015Article
- NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins.Nucleic acids research · 2014Article
- The FF4 and FF5 domains of transcription elongation regulator 1 (TCERG1) target proteins to the periphery of speckles.The Journal of biological chemistry · 2012Article
- Nuclear speckles.Cold Spring Harbor perspectives in biology · 2011Review
- Dimerization and a novel Tax speckled structure localization signal are required for Tax nuclear localization.Journal of virology · 2009Article
- Genome-wide analysis of histidine repeats reveals their role in the localization of human proteins to the nuclear speckles compartment.PLoS genetics · 2009Article
- Analysis of influenza B Virus NS1 protein trafficking reveals a novel interaction with nuclear speckle domains.Journal of virology · 2009Article
- Different requirements of the kinase and UHM domains of KIS for its nuclear localization and binding to splicing factors.Journal of molecular biology · 2008Article
- Altered localization of retinoid X receptor alpha coincides with loss of retinoid responsiveness in human breast cancer MDA-MB-231 cells.Molecular and cellular biology · 2004Article
- The spatial targeting and nuclear matrix binding domains of SRm160.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A novel splicing regulator shares a nuclear import pathway with SR proteins.The EMBO journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SF3b(155) is an essential spliceosomal protein, highly conserved during evolution. It has been identified as a subunit of splicing factor SF3b, which, together with a second multimeric complex termed SF3a, interacts specifically with the 12S U2 snRNP and converts it into the active 17S form. The protein displays a characteristic intranuclear localization. It is diffusely distributed in the nucleoplasm but highly concentrated in defined intranuclear structures termed "speckles," a subnuclear compartment enriched in small ribonucleoprotein particles and various splicing factors. The primary sequence of SF3b(155) suggests a multidomain structure, different from those of other nuclear speckles components. To identify which part of SF3b(155) determines its specific intranuclear localization, we have constructed expression vectors encoding a series of epitope-tagged SF3b(155) deletion mutants as well as chimeric combinations of SF3b(155) sequences with the soluble cytoplasmic protein pyruvate kinase. Following transfection of cultured mammalian cells, we have identified (i) a functional nuclear localization signal of the monopartite type (KRKRR, amino acids 196--200) and (ii) a molecular segment with multiple threonine-proline repeats (amino acids 208--513), which is essential and sufficient to confer a specific accumulation in nuclear speckles. This latter sequence element, in particular amino acids 208--440, is required for correct subcellular localization of SF3b(155) and is also sufficient to target a reporter protein to nuclear speckles. Moreover, this "speckle-targeting sequence" transfers the capacity for interaction with other U2 snRNP components.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.