ArticleJournal of bacteriology2001
The hetF gene product is essential to heterocyst differentiation and affects HetR function in the cyanobacterium Nostoc punctiforme.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 89 citations in OpenAlex.
- Hacking heterocysts: advances in the genetic regulation of heterocyst differentiation.Archives of microbiology · 2025Review
- Metatranscriptomics reveals gene expression dynamics during an anatoxin-a producing Dolichospermum bloom in a western coastal lake.Chemosphere · 2025Article
- Tradeoffs between phage resistance and nitrogen fixation drive the evolution of genes essential for cyanobacterial heterocyst functionality.The ISME journal · 2024Article
- PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc sp. PCC 7120.Science China. Life sciences · 2023Article
- Photobiohydrogen Production and Strategies for HAdvances in biochemical engineering/biotechnology · 2023Article
- A proteolytic pathway coordinates cell division and heterocyst differentiation in the cyanobacteriumProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Interaction network among factors involved in heterocyst-patterning in cyanobacteria.Molecular genetics and genomics : MGG · 2022Article
- The Heterocyst-Specific Small RNA NsiR1 Regulates the Commitment to Differentiation in Nostoc.Microbiology spectrum · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Genetic and lipidomic analyses suggest thatAccess microbiology · 2022Article
- HetF Protein Is a New Divisome Component in a Filamentous and Developmental Cyanobacterium.mBio · 2021Article
- Perspectives on Endosymbiosis in Coralloid Roots: Association of Cycads and Cyanobacteria.Frontiers in microbiology · 2019Review
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulatory RNAs in photosynthetic cyanobacteria.FEMS microbiology reviews · 2015Review
- Subcellular localization and clues for the function of the HetN factor influencing heterocyst distribution in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2014Article
- Article
- Riboregulators and the role of Hfq in photosynthetic bacteria.RNA biology · 2014Review
- Variations in the rhythms of respiration and nitrogen fixation in members of the unicellular diazotrophic cyanobacterial genus Cyanothece.Plant physiology · 2013Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel gene, hetF, was identified as essential for heterocyst development in the filamentous cyanobacterium Nostoc punctiforme strain ATCC 29133. In the absence of combined nitrogen, hetF mutants were unable to differentiate heterocysts, whereas extra copies of hetF in trans induced the formation of clusters of heterocysts. Sequences hybridizing to a hetF probe were detected only in heterocyst-forming cyanobacteria. The inactivation and multicopy effects of hetF were similar to those of hetR, which encodes a self-degrading serine protease thought to be a central regulator of heterocyst development. Increased transcription of hetR begins in developing cells 3 to 6 h after deprivation for combined nitrogen (N step-down), and the HetR protein specifically accumulates in heterocysts. In the hetF mutant, this increase in hetR transcription was delayed, and a hetR promoter::green fluorescent protein (GFP) transcriptional reporter indicated that increased transcription of hetR occurred in all cells rather than only in developing heterocysts. When a fully functional HetR-GFP fusion protein was expressed in the hetF mutant from a multicopy plasmid, HetR-GFP accumulated nonspecifically in all cells under nitrogen-replete conditions; when expressed in the wild type, HetR-GFP was observed only in heterocysts after N step-down. HetF therefore appears to cooperate with HetR in a positive regulatory pathway and may be required for the increased transcription of hetR and localization of the HetR protein in differentiating heterocysts.
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