ArticleJournal of bacteriology2001
GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives.Molecules (Basel, Switzerland) · 2022Review
- Review
- Characterization and Inhibition of 1-Deoxy-d-Xylulose 5-Phosphate Reductoisomerase: A Promising Drug Target inACS infectious diseases · 2021Article
- SEED CAROTENOID DEFICIENT Functions in Isoprenoid Biosynthesis via the Plastid MEP Pathway.Plant physiology · 2019Article
- A high-throughput screening campaign to identify inhibitors of DXP reductoisomerase (IspC) and MEP cytidylyltransferase (IspD).Analytical biochemistry · 2018Article
- New Insight into Isoprenoids Biosynthesis Process and Future Prospects for Drug Designing in Plasmodium.Frontiers in microbiology · 2016Review
- Mechanistic aspects of carotenoid biosynthesis.Chemical reviews · 2014Review
- Article
- Methylerythritol phosphate pathway of isoprenoid biosynthesis.Annual review of biochemistry · 2013Review
- Cross-complementation study of the flagellar type III export apparatus membrane protein FlhB.PloS one · 2012Article
- Biochemistry of the non-mevalonate isoprenoid pathway.Cellular and molecular life sciences : CMLS · 2011Review
- Francisella tularensis 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase: kinetic characterization and phosphoregulation.PloS one · 2011Article
- The nonmevalonate pathway of isoprenoid biosynthesis in Mycobacterium tuberculosis is essential and transcriptionally regulated by Dxs.Journal of bacteriology · 2010Article
- Article
- The Mycobacterium tuberculosis MEP (2C-methyl-d-erythritol 4-phosphate) pathway as a new drug target.Tuberculosis (Edinburgh, Scotland) · 2009Review
- Practical and theoretical advances in predicting the function of a protein by its phylogenetic distribution.Journal of the Royal Society, Interface · 2008Review
- Identification of novel virulence determinants in Mycobacterium paratuberculosis by screening a library of insertional mutants.Infection and immunity · 2006Article
- Lethal mutations in the isoprenoid pathway of Salmonella enterica.Journal of bacteriology · 2006Article
- Regulation of Mycobacterium tuberculosis cell envelope composition and virulence by intramembrane proteolysis.Nature · 2005Article
- Functional analysis of the final steps of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway to isoprenoids in plants using virus-induced gene silencing.Plant physiology · 2004Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. In this work, we demonstrate that the gcpE gene of Escherichia coli is involved in this pathway. E. coli cells were genetically engineered to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. These cells were then deleted for the essential gcpE gene and were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of gcpE.
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