ArticleProtein science : a publication of the Protein Society2001
Coupling of antibodies via protein Z on modified polyoma virus-like particles.
Article in Protein science : a publication of the Protein Society, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 50 citations in OpenAlex.
- Translational Challenges and Prospective Solutions in the Implementation of Biomimetic Delivery Systems.Pharmaceutics · 2023Review
- Protein cage assembly across multiple length scales.Chemical Society reviews · 2018Review
- Biomedical and Catalytic Opportunities of Virus-Like Particles in Nanotechnology.Advances in virus research · 2017Review
- Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles.Scientific reports · 2016Article
- Intracellular targeting with engineered proteins.F1000Research · 2016Review
- A plant derived multifunctional tool for nanobiotechnology based on Tomato bushy stunt virus.Transgenic research · 2013Article
- Relevant uses of surface proteins--display on self-organized biological structures.Microbial biotechnology · 2012Review
- Engineered biological entities for drug delivery and gene therapy protein nanoparticles.Progress in molecular biology and translational science · 2011Review
- Viral nanoparticles as macromolecular devices for new therapeutic and pharmaceutical approaches.International journal of physiology, pathophysiology and pharmacology · 2010Article
- Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells.Virus genes · 2007Article
- Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Formation of polyomavirus-like particles with different VP1 molecules that bind the urokinase plasminogen activator receptor.Journal of virology · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Therapeutic application of virus-based delivery systems often implies a change of the tropism of these vectors. This can be achieved by insertion of polypeptides (e.g., antibody fragments) in viral coat proteins. Such fusion proteins have only been used in viral vectors so far and, as part of a virus, they have not been available for a detailed biophysical characterization. We analyzed a fusion protein called VP1-Z, which is based on the polyoma virus coat protein VP1 and protein Z. Protein Z is an engineered antibody-binding domain derived from protein A from Staphylococcus aureus. The fusion VP1-Z was constructed by insertion of protein Z in the HI-loop of VP1. As wild-type VP1, VP1-Z formed pentameric capsomers and assembled to VLPs in vitro. The stability of these particles was very similar compared to that of VLPs of wild-type VP1. Protein Z was fully structured in the fusion protein and was still capable of binding antibodies on the surface of VLPs of VP1-Z. Using this fusion protein, we could change the tropism of polyoma VLPs toward cells presenting on their surface the antigen of the coupled antibody.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.