ArticleProtein science : a publication of the Protein Society2001
Understanding thermostability in cytochrome P450 by combinatorial mutagenesis.
Article in Protein science : a publication of the Protein Society, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Differential effects of lipid composition on the thermal and functional properties of membrane associated CYP2J2.Protein science : a publication of the Protein Society · 2026Article
- Functions of ROS in Macrophages and Antimicrobial Immunity.Antioxidants (Basel, Switzerland) · 2021Review
- Characterization of a thermophilic cytochrome P450 of the CYP203A subfamily from Binh Chau hot spring in Vietnam.FEBS open bio · 2021Article
- Article
- Comparative Proteomics of Salt-Tolerant and Salt-Sensitive Maize Inbred Lines to Reveal the Molecular Mechanism of Salt Tolerance.International journal of molecular sciences · 2019Article
- Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119.Bioinorganic chemistry and applications · 2019Article
- Filling the Void: Introducing Aromatic Interactions into Solvent Tunnels To Enhance Lipase Stability in Methanol.Applied and environmental microbiology · 2018Article
- Entropic contribution to enhanced thermal stability in the thermostable P450 CYP119.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Using molecular dynamics to probe the structural basis for enhanced stability in thermal stable cytochromes P450.Biochemistry · 2010Article
- Kinetics and activation parameters for oxidations of styrene by Compounds I from the cytochrome P450(BM-3) (CYP102A1) heme domain and from CYP119.Biochemistry · 2009Article
- Quantitative production of compound I from a cytochrome P450 enzyme at low temperatures. Kinetics, activation parameters, and kinetic isotope effects for oxidation of benzyl alcohol.Journal of the American Chemical Society · 2009Article
- Kinetics of oxidation of benzphetamine by compounds I of cytochrome P450 2B4 and its mutants.Journal of the American Chemical Society · 2009Article
- Kinetic isotope effects in hydroxylation reactions effected by cytochrome P450 compounds I implicate multiple electrophilic oxidants for P450-catalyzed oxidations.Biochemistry · 2009Article
- Spectra and kinetic studies of the compound I derivative of cytochrome P450 119.Journal of the American Chemical Society · 2008Article
- X-ray absorption spectroscopic characterization of a cytochrome P450 compound II derivative.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Low-frequency dynamics of Caldariomyces fumago chloroperoxidase probed by femtosecond coherence spectroscopy.Biochemistry · 2008Article
- A localized specific interaction alters the unfolding pathways of structural homologues.Journal of the American Chemical Society · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The cytochromes P450 are an important class of mono-oxygenases involved in xenobiotic metabolism and steroid biosynthesis in a diverse set of life forms. Discovery of CYP-119, a P450 from the archea Sulfolobus solfataricus has provided a means for understanding nature's method of stabilizing this important protein superfamily. To identify classes of stabilizing interactions used by CYP-119, we have generated a randomized library of point mutants and screened for mutants that are less thermostable than the wild type by monitoring the characteristic Soret band in the visible region of the cell lysis. The selected mutants were characterized by differential scanning calorimetry to compare the temperatures of the melting transitions of the various mutants. The identified mutations suggested that electrostatic interactions involving salt links and charge-charge interactions, as well as contributions from other interactions such as aromatic stacking, and side chain volume of hydrophobic residues contribute to enhanced thermostability in this cytochrome P450.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.