ArticleNucleic acids research2001
Manufacturing DNA microarrays of high spot homogeneity and reduced background signal.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 226 citations in OpenAlex.
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- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- An equipment-free polydimethylsiloxane microfluidic spotter for fabrication of microarrays.Biomicrofluidics · 2014Article
- Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays.BMC biophysics · 2013Article
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- An gene expression pattern.Journal of natural science, biology, and medicine · 2012Article
- Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development.Biosensors & bioelectronics · 2011Article
- Facile DNA immobilization on surfaces through a catecholamine polymer.Angewandte Chemie (International ed. in English) · 2011Article
- Multiplexed, rapid point of care platform to quantify allergen-specific IgE.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2011Article
- Differential expression of presynaptic genes in a rat model of postnatal hypoxia: relevance to schizophrenia.European archives of psychiatry and clinical neuroscience · 2010Article
- Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome.BMC research notes · 2010Article
- Phosphate-dependent behavior of the archaeon Halobacterium salinarum strain R1.Journal of bacteriology · 2009Article
- Sildenafil augments early protective transcriptional changes after ischemia in mouse myocardium.Gene · 2009Article
- Development and application of a microarray meter tool to optimize microarray experiments.BMC research notes · 2008Article
- Genotypic resistance testing in HIV by arrayed primer extension.Analytical and bioanalytical chemistry · 2008Article
- Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases.Nucleic acids research · 2008Article
- Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.PloS one · 2008Article
- DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.Journal of bacteriology · 2008Article
- Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays.PloS one · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyses on DNA microarrays depend considerably on spot quality and a low background signal of the glass support. By using betaine as an additive to a spotting solution made of saline sodium citrate, both the binding efficiency of spotted PCR products and the homogeneity of the DNA spots is improved significantly on aminated surfaces such as glass slides coated with the widely used poly-L-lysine or aminosilane. In addition, non-specific background signal is markedly diminished. Concomitantly, during the arraying procedure, the betaine reduces evaporation from the microtitre dish wells, which hold the PCR products. Subsequent blocking of the chip surface with succinic anhydride was improved considerably in the presence of the non-polar, non-aqueous solvent 1,2-dichloroethane and the acylating catalyst N:-methylimidazole. This procedure prevents the overall background signal that occurs with the frequently applied aqueous solvent 1-methyl-2-pyrrolidone in borate buffer because of DNA that re-dissolves from spots during the blocking process, only to bind again across the entire glass surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.