ArticleNucleic acids research2001
Detection of mitochondrial single nucleotide polymorphisms using a primer elongation reaction on oligonucleotide microarrays.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 84 citations in OpenAlex.
- Article
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- Biological and nanotechnological applications using interactions between ionic liquids and nucleic acids.Biophysical reviews · 2018Review
- TGFA and IRF6 contribute to the risk of nonsyndromic cleft lip with or without cleft palate in northeast China.PloS one · 2013Article
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
- Visual detection of single-base mismatches in DNA using hairpin oligonucleotide with double-target DNA binding sequences and gold nanoparticles.Biosensors & bioelectronics · 2012Article
- The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE.BMC plant biology · 2011Article
- Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation.Molecules (Basel, Switzerland) · 2009Article
- Product length, dye choice, and detection chemistry in the bead-emulsion amplification of millions of single DNA molecules in parallel.Analytical chemistry · 2009Article
- Quantitative evaluation of oligonucleotide surface concentrations using polymerization-based amplification.Analytical and bioanalytical chemistry · 2008Article
- Sensitive detection of SARS coronavirus RNA by a novel asymmetric multiplex nested RT-PCR amplification coupled with oligonucleotide microarray hybridization.Methods in molecular medicine · 2005Article
- Sequence polymorphisms within the human mitochondrial genes MTATP6, MTATP8 and MTND4.International journal of legal medicine · 2003Article
- SNP typing by apyrase-mediated allele-specific primer extension on DNA microarrays.Nucleic acids research · 2002Article
- DNA microarrays with PAMAM dendritic linker systems.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel allele-specific primer elongation protocol using a DNA polymerase on oligonucleotide chips. Oligonucleotide primers carrying polymorphic sites at their free 3'end were covalently bound to glass slides. The generation of single-stranded targets of genomic DNA containing single nuclotide polymorphisms (SNPs) to be typed was achieved by an asymmetric PCR reaction or exonuclease treatment of phosphothioate (PTO)-modified PCR products. In the presence of DNA polymerase and all four dNTPs, with Cy3-dUTP replacing dTTP, allele-specific extension of the immobilized primers took place along a stretch of target DNA sequence. The yield of elongated products was increased by repeated reaction cycles. We performed multiplexed assays with many small DNA targets, or used single targets of up to 4.4 kb mitochondrial DNA (mtDNA) sequence to detect multiple SNPs in one reaction. The latter approach greatly simplifies preamplification of SNP-containing regions, thereby providing a framework for typing hundreds of mtDNA polymorphisms.
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