ArticleNucleic acids research2001
The length of telomeric G-rich strand 3'-overhang measured by oligonucleotide ligation assay.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- A CRISPR/Cas9-induced blunt-end telomere system in S. pombe reveals RNase H2-dependent RNA primer removal at the terminal Okazaki fragment of lagging telomeres.Nucleic acids research · 2026Article
- Effects of ribonucleotides on telomeric G4 formation, dynamics, and initiation of ribonucleotide excision repair by RNase H2.Nucleic acids research · 2026Article
- Base excision repair within structure-forming repeat sequences and its impact on cancer and other diseases.NAR cancer · 2025Review
- Methods that shaped telomerase research.Biogerontology · 2024Review
- Single-Run Catalysis and Kinetic Control of Human Telomerase Holoenzyme.Advances in experimental medicine and biology · 2022Review
- Decoding the Structural Dynamics and Conformational Alternations of DNA Secondary Structures by Single-Molecule FRET Microspectroscopy.Frontiers in molecular biosciences · 2021Review
- Ensemble Sensing Using Single-Molecule DNA Copolymers.Analytical chemistry · 2020Article
- G-Quadruplexes at Telomeres: Friend or Foe?Molecules (Basel, Switzerland) · 2020Review
- Position-Dependent Effect of Guanine Base Damage and Mutations on Telomeric G-Quadruplex and Telomerase Extension.Biochemistry · 2020Article
- Shelterin Complex at Telomeres: Implications in Ageing.Clinical interventions in aging · 2020Review
- Review
- Telomerase and Telomeres in Endometrial Cancer.Frontiers in oncology · 2019Review
- Random Formation of G-Quadruplexes in the Full-Length Human Telomere Overhangs Leads to a Kinetic Folding Pattern with Targetable Vacant G-Tracts.Biochemistry · 2018Article
- Article
- Molecular mechanisms by which oxidative DNA damage promotes telomerase activity.Nucleic acids research · 2017Article
- Implications of telomeres and telomerase in endometrial pathology.Human reproduction update · 2017Review
- TRF1 and TRF2 binding to telomeres is modulated by nucleosomal organization.Nucleic acids research · 2015Article
- Hydrodynamic Models of G-Quadruplex Structures.Methods in enzymology · 2015Article
- Tel1 and Rad51 are involved in the maintenance of telomeres with capping deficiency.Nucleic acids research · 2013Article
- HnRNP A1 phosphorylated by VRK1 stimulates telomerase and its binding to telomeric DNA sequence.Nucleic acids research · 2012Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mechanism of long-tail formation, the presence and the role of these structures in telomere maintenance or shortening are not completely understood. In a search for a simple method to accurately measure the 3'-overhang we have established a protocol based on the ligation of telomeric oligonucleotide hybridized to non-denatured DNA under stringent conditions (oligonucleotide ligation assay with telomeric repeat oligonucleotide). This method enabled us to detect a large proportion of G-rich single-stranded telomeric DNA that was as short as 24 nt. Nevertheless, we showed G-tails longer than 400 nt. In all tested cells the lengths ranging from 108 to 270 nt represented only 37% of the whole molecule population, while 56-62% were <90 nt. Our protocol provides a simple and sensitive method for measuring the length of naturally occurring unpaired repeated DNA.
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