ArticleNucleic acids research2001
A single alteration 20 nt 5' to an editing target inhibits chloroplast RNA editing in vivo.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids.Nucleic acids research · 2012Article
- A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites.The Plant cell · 2009Article
- Loss of matK RNA editing in seed plant chloroplasts.BMC evolutionary biology · 2009Article
- A comparative genomics approach identifies a PPR-DYW protein that is essential for C-to-U editing of the Arabidopsis chloroplast accD transcript.RNA (New York, N.Y.) · 2009Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Amino acid sequence variations in Nicotiana CRR4 orthologs determine the species-specific efficiency of RNA editing in plastids.Nucleic acids research · 2008Article
- Characteristics and prediction of RNA editing sites in transcripts of the Moss Takakia lepidozioides chloroplast.DNA research : an international journal for rapid publication of reports on genes and genomes · 2008Article
- Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets.The Journal of biological chemistry · 2008Article
- Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts.Nucleic acids research · 2008Article
- Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- RNA editing site recognition in heterologous plant mitochondria.Current genetics · 2006Article
- Maintenance of plastid RNA editing activities independently of their target sites.EMBO reports · 2006Article
- Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro.Nucleic acids research · 2006Article
- An in vitro RNA editing system from cauliflower mitochondria: editing site recognition parameters can vary in different plant species.RNA (New York, N.Y.) · 2005Article
- Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts.Nucleic acids research · 2005Article
- A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcripts.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Complex cis-elements determine an RNA editing site in pea mitochondria.Nucleic acids research · 2004Article
- Eukaryotic genome evolution: rearrangement and coevolution of compartmentalized genetic information.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2003Review
- Cross-competition in transgenic chloroplasts expressing single editing sites reveals shared cis elements.Molecular and cellular biology · 2002Article
- Recognition of RNA editing sites is directed by unique proteins in chloroplasts: biochemical identification of cis-acting elements and trans-acting factors involved in RNA editing in tobacco and pea chloroplasts.Molecular and cellular biology · 2002Article
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3 authors.
Funding
Abstract
Transcripts of typical dicot plant plastid genes undergo C-->U RNA editing at approximately 30 locations, but there is no consensus sequence surrounding the C targets of editing. The cis-acting elements required for editing of the C located at tobacco rpoB editing site II were investigated by introducing translatable chimeric minigenes containing sequence -20 to +6 surrounding the C target of editing. When the -20 to +6 sequence specified by the homologous region present in the black pine chloroplast genome was incorporated, virtually no editing of the transcripts occurred in transgenic tobacco plastids. Nucleotides that differ between the black pine and tobacco sequence were tested for their role in C-->U editing by designing chimeric genes containing one or more of these divergent nucleotides. Surprisingly, the divergent nucleotide that had the strongest negative effect on editing of the minigene transcript was located -20 nt 5' to the C target of editing. Expression of transgene transcripts carrying the 27 nt sequence did not affect the editing extent of the endogenous rpoB transcripts, even though the chimeric transcripts were much more abundant than those of the endogenous gene. In plants carrying a 93 nt rpoB editing site sequence, transgene transcripts accumulated to a level three times greater than transgene transcripts in the plants carrying the 27 nt rpoB editing sites and resulted in editing of the endogenous transcripts from 100 to 50%. Both a lower affinity of the 27 nt site for a trans-acting factor and lower abundance of the transcript could explain why expression of minigene transcripts containing the 27 nt sequence did not affect endogenous editing.
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