ArticleThe Journal of cell biology2001
Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 118 citations in OpenAlex.
- Mechanisms of nuclear segregation in a multinucleate multibudding yeast.The Journal of cell biology · 2025Article
- Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.PLoS biology · 2025Article
- Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.The Journal of cell biology · 2024Article
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- Temporal control of contact site formation reveals a relationship between mitochondrial division and Num1-mediated mitochondrial tethering.Molecular biology of the cell · 2023Article
- Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.Journal of cell science · 2022Article
- Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.Molecular biology of the cell · 2022Article
- The Expanding and Unexpected Functions of Mitochondria Contact Sites.Trends in cell biology · 2019Review
- An essential contractile ring protein controls cell division in Plasmodium falciparum.Nature communications · 2019Article
- A conserved mechanism for mitochondria-dependent dynein anchoring.Molecular biology of the cell · 2019Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1.eLife · 2018Article
- Mitochondrial anchors: Positioning mitochondria and more.Biochemical and biophysical research communications · 2018Review
- The role of mitochondria in anchoring dynein to the cell cortex extends beyond clustering the anchor protein.Cell cycle (Georgetown, Tex.) · 2018Article
- A nuclear contortionist: the mitotic migration ofMycology · 2018Review
- Article
- Mitochondria-driven assembly of a cortical anchor for mitochondria and dynein.The Journal of cell biology · 2017Article
- LTE1 promotes exit from mitosis by multiple mechanisms.Molecular biology of the cell · 2016Article
- Num1 anchors mitochondria to the plasma membrane via two domains with different lipid binding specificities.The Journal of cell biology · 2016Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Num1p, a cortical 313-kD protein, controls cytoplasmic microtubule (cMT) functions and nuclear migration through the bud neck in anaphase cells. A green fluorescent protein (GFP)-Num1p fusion protein localizes at the bud tip and the distal mother pole of living cells, apparently forming cMT capture sites at late anaphase. In addition, galactose-induced GFP-Num1p is seen at the bud neck and in lateral regions of the mother cortex. The bud tip location of Num1p depends on Bni1p but does not require Kar9p, Dyn1p, or cMTs, whereas cMT contacts with polar Num1p dots are reduced in cells lacking Dyn1p. Num1p associates with the dynein intermediate chain Pac11p in the presence of Dyn1p, and with the alpha-tubulin Tub3p, as shown by coimmune precipitation of tagged proteins. Num1p also forms a complex with Bni1p and Kar9p, although Num1p is not required for Bni1p- and Kar9p-dependent nuclear migration to the bud neck in preanaphase cells. Our data suggest that Num1p controls nuclear migration during late anaphase by forming dynein-interacting cortical cMT capture sites at both cellular poles. In addition, Num1p may transiently cooperate with an associated Bni1p-Kar9p complex at the bud tip of early anaphase cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.