ArticleJournal of virology2001
Nef-induced CD4 downregulation: a diacidic sequence in human immunodeficiency virus type 1 Nef does not function as a protein sorting motif through direct binding to beta-COP.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- How HIV Nef Proteins Hijack Membrane Traffic To Promote Infection.Journal of virology · 2019Review
- Article
- AP-2 Is the Crucial Clathrin Adaptor Protein for CD4 Downmodulation by HIV-1 Nef in Infected Primary CD4+ T Cells.Journal of virology · 2015Article
- Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor.The Journal of biological chemistry · 2014Article
- Elucidating novel hepatitis C virus-host interactions using combined mass spectrometry and functional genomics approaches.Molecular & cellular proteomics : MCP · 2014Article
- The activity of Nef on HIV-1 infectivity.Frontiers in microbiology · 2014Review
- HIV-1 Nef inhibits Protease activity and its absence alters protein content of mature viral particles.PloS one · 2014Article
- Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.Journal of virology · 2012Article
- HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals.AIDS research and human retroviruses · 2011Article
- HIV immune evasion disruption of antigen presentation by the HIV Nef protein.Advances in virus research · 2011Review
- Nef-induced CD4 endocytosis in human immunodeficiency virus type 1 host cells: role of p56lck kinase.Journal of virology · 2009Article
- Human immunodeficiency virus type 1 Nef protein targets CD4 to the multivesicular body pathway.Journal of virology · 2009Article
- HIV-1 Nef targets MHC-I and CD4 for degradation via a final common beta-COP-dependent pathway in T cells.PLoS pathogens · 2008Article
- Article
- Article
- Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor.Journal of virology · 2007Article
- Intra- and intersubtype alternative Pak2-activating structural motifs of human immunodeficiency virus type 1 Nef.Journal of virology · 2006Article
- Human immunodeficiency virus type 1 Nef: adapting to intracellular trafficking pathways.Microbiology and molecular biology reviews : MMBR · 2006Review
- A hydrophobic binding surface on the human immunodeficiency virus type 1 Nef core is critical for association with p21-activated kinase 2.Journal of virology · 2006Article
- Dynamic evolution of the human immunodeficiency virus type 1 pathogenic factor, Nef.Journal of virology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The Nef protein from the human immunodeficiency virus (HIV) induces CD4 cell surface downregulation by interfering with the endocytic machinery. It has been recently proposed that binding of HIV type 1 Nef to the beta subunit of COPI coatomers participated in the Nef-induced CD4 downregulation through recognition of a novel diacidic motif found in the C-terminal disordered loop of Nef (V. Piguet, F. Gu, M. Foti, N. Demaurex, J. Gruenberg, J. L. Carpentier, and D. Trono, Cell 97:63-73, 1999). We have mutated the glutamate residues which formed this motif in order to document this observation. Surprisingly, mutation of the diacidic sequence of Nef did not significantly affect its ability (i) to interact with beta-COP, (ii) to downregulate CD4 cell surface expression, and (iii) to address an integral resident membrane protein containing Nef as the cytoplasmic domain to the endocytic pathway. Our results indicate that these acidic residues are not involved in the connection of Nef with the endocytic machinery through binding to beta-COP. Additional studies are thus required to characterize the residues of Nef involved in the binding to beta-COP and to evaluate the contribution of this interaction to the Nef-induced perturbations of membrane trafficking.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.