ArticleJournal of virology2001
Genetic analysis of a poliovirus/hepatitis C virus chimera: new structure for domain II of the internal ribosomal entry site of hepatitis C virus.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Isolation and Identification of Inter-SpeciesViruses · 2021Article
- Hepatitis C Virus Translation Regulation.International journal of molecular sciences · 2020Review
- HCVIVdb: The hepatitis-C IRES variation database.BMC microbiology · 2016Article
- HMGB1 Promotes Hepatitis C Virus Replication by Interaction with Stem-Loop 4 in the Viral 5' Untranslated Region.Journal of virology · 2015Article
- Conserved RNA secondary structures and long-range interactions in hepatitis C viruses.RNA (New York, N.Y.) · 2015Article
- HCV IRES interacts with the 18S rRNA to activate the 40S ribosome for subsequent steps of translation initiation.Nucleic acids research · 2013Article
- A twist in the tail: SHAPE mapping of long-range interactions and structural rearrangements of RNA elements involved in HCV replication.Nucleic acids research · 2012Article
- Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome.Journal of virology · 2011Article
- Regulation of PKR by HCV IRES RNA: importance of domain II and NS5A.Journal of molecular biology · 2010Article
- Inhibitor-induced structural change in the HCV IRES domain IIa RNA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Virus attenuation by genome-scale changes in codon pair bias.Science (New York, N.Y.) · 2008Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- Reduction of the rate of poliovirus protein synthesis through large-scale codon deoptimization causes attenuation of viral virulence by lowering specific infectivity.Journal of virology · 2006Article
- Coordinated assembly of human translation initiation complexes by the hepatitis C virus internal ribosome entry site RNA.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Replication of poliovirus RNA with complete internal ribosome entry site deletions.Journal of virology · 2004Article
- Long-range RNA-RNA interaction between the 5' nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation.RNA (New York, N.Y.) · 2003Article
- Role of the 5'-proximal stem-loop structure of the 5' untranslated region in replication and translation of hepatitis C virus RNA.Journal of virology · 2003Article
- Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction.RNA (New York, N.Y.) · 2002Article
- Mutation Master: profiles of substitutions in hepatitis C virus RNA of the core, alternate reading frame, and NS2 coding regions.RNA (New York, N.Y.) · 2002Article
- Sequences in the 5' nontranslated region of hepatitis C virus required for RNA replication.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Internal ribosomal entry sites (IRESs) of certain plus-strand RNA viruses direct cap-independent initiation of protein synthesis both in vitro and in vivo, as can be shown with artificial dicistronic mRNAs or with chimeric viral genomes in which IRES elements were exchanged from one virus to another. Whereas IRESs of picornaviruses can be readily analyzed in the context of their cognate genome by genetics, the IRES of hepatitis C virus (HCV), a Hepacivirus belonging to Flaviviridae, cannot as yet be subjected to such analyses because of difficulties in propagating HCV in tissue culture or in experimental animals. This enigma has been overcome by constructing a poliovirus (PV) whose translation is controled by the HCV IRES. Within the PV/HCV chimera, the HCV IRES has been subjected to systematic 5' deletion analyses to yield a virus (P/H710-d40) whose replication kinetics match that of the parental poliovirus type 1 (Mahoney). Genetic analyses of the HCV IRES in P/H710-d40 have confirmed that the 5' border maps to domain II, thereby supporting the validity of the experimental approach applied here. Additional genetic experiments have provided evidence for a novel structural region within domain II. Arguments that the phenotypes observed with the mutant chimera relate solely to impaired genome replication rather than deficiencies in translation have been dispelled by constructing novel dicistronic poliovirus replicons with the gene order [PV]cloverleaf-[HCV]IRES-Deltacore-R-Luc-[PV]IRES-F-Luc-P2,3-3'NTR, which have allowed the measurement of HCV IRES-dependent translation independently from the replication of the replicon RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.