ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Multiple distinct signal pathways, including an autocrine neurotrophic mechanism, contribute to the survival-promoting effect of depolarization on spiral ganglion neurons in vitro.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 134 citations in OpenAlex.
- Tissue engineering strategies for spiral ganglion neuron protection and regeneration.Journal of nanobiotechnology · 2024Review
- Candidate Key Proteins in Tinnitus: A Bioinformatic Study of Synaptic Transmission in Spiral Ganglion Neurons.Cellular and molecular neurobiology · 2023Article
- Amplification of input differences by dynamic heterogeneity in the spiral ganglion.Journal of neurophysiology · 2022Article
- Opposite Roles of NT-3 and BDNF in Synaptic Remodeling of the Inner Ear Induced by Electrical Stimulation.Cellular and molecular neurobiology · 2021Article
- Dynamic Heterogeneity Shapes Patterns of Spiral Ganglion Activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Interaction of micropatterned topographical and biochemical cues to direct neurite growth from spiral ganglion neurons.Hearing research · 2021Article
- Pleiotrophin increases neurite length and number of spiral ganglion neurons in vitro.Experimental brain research · 2019Article
- AAV-Mediated Neurotrophin Gene Therapy Promotes Improved Survival of Cochlear Spiral Ganglion Neurons in Neonatally Deafened Cats: Comparison of AAV2-hBDNF and AAV5-hGDNF.Journal of the Association for Research in Otolaryngology : JARO · 2019Article
- Stem Cell Based Drug Delivery for Protection of Auditory Neurons in a Guinea Pig Model of Cochlear Implantation.Frontiers in cellular neuroscience · 2019Article
- CaBP1 regulates CaMolecular and cellular neurosciences · 2018Article
- Photopolymerized micropatterns with high feature frequencies overcome chemorepulsive borders to direct neurite growth.Journal of tissue engineering and regenerative medicine · 2018Article
- Photopolymerized Microfeatures Guide Adult Spiral Ganglion and Dorsal Root Ganglion Neurite Growth.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2018Article
- Article
- Effects of brain-derived neurotrophic factor (BDNF) on the cochlear nucleus in cats deafened as neonates.Hearing research · 2016Article
- Intracellular calcium and cyclic nucleotide levels modulate neurite guidance by microtopographical substrate features.Journal of biomedical materials research. Part A · 2016Article
- AP-1 Transcription Factors Mediate BDNF-Positive Feedback Loop in Cortical Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Microtopographical features generated by photopolymerization recruit RhoA/ROCK through TRPV1 to direct cell and neurite growth.Biomaterials · 2015Article
- Enhanced bioavailability of nerve growth factor with phytantriol lipid-based crystalline nanoparticles in cochlea.International journal of nanomedicine · 2015Article
- Material stiffness effects on neurite alignment to photopolymerized micropatterns.Biomacromolecules · 2014Article
- Inhibition of MMP-2 but not MMP-9 influences inner ear spiral ganglion neurons in vitro.Cellular and molecular neurobiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have shown previously that BDNF, neurotrophin-3 (NT-3), chlorphenylthio-cAMP (cpt-cAMP) (a permeant cAMP analog), and membrane depolarization promote spiral ganglion neuron (SGN) survival in vitro in an additive manner, depolarization having the greatest efficacy. Expression of both BDNF and of NT-3 is detectable in cultured SGNs after plating in either depolarizing or nondepolarizing medium. These neurotrophins promote survival by an autocrine mechanism; TrkB-IgG or TrkC-IgG, which block neurotrophin binding to, respectively, TrkB and TrkC, partially inhibit the trophic effect of depolarization. The mitogen-activated protein kinase kinase inhibitor PD98059 and the phosphatidylinositol-3-OH kinase inhibitor LY294002 both abolish trophic support by neurotrophins but only partially inhibit support by depolarization. Inhibition by these compounds is not additive with inhibition by Trk-IgGs. The cAMP antagonist Rp-adenosine-3',5'-cyclic-phosphorothioate (Rp-cAMPS) abolishes survival attributable to cpt-cAMP but has no effect on that attributable to neurotrophins, nor do inhibitors of neurotrophin-dependent survival affect survival attributable to cpt-cAMP. However, Rp-cAMPS does partially inhibit depolarization-dependent survival, an inhibition that is additive with that by Trk-IgGs, PD98059, or LY294002. Moreover, Rp-cAMPS prevents depolarization-dependent survival of PC12 cells maintained in subthreshold levels of NGF. Inhibition of Ca(2+)/calmodulin-dependent protein kinases (CaMKs) with KN-62 reduces SGN survival independently of Rp-cAMPS, Trk-IgGs, and LY294002 and additively with them. Combined inhibition of Trk, cAMP, and CaMK signaling prevents depolarization-dependent survival. Thus, survival of SGNs under depolarizing conditions involves additivity among a depolarization-independent autocrine pathway, a cAMP-dependent pathway, and a CaMK-dependent pathway.
Indexed as
Identifiers
11264301PMC6762385W1675685854What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.