ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 110 citations in OpenAlex.
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Kynurenines and Neurofilament Light Chain in Multiple Sclerosis.Frontiers in neuroscience · 2021Review
- A possible mechanism for neurofilament slowing down in myelinated axon: Phosphorylation-induced variation of NF kinetics.PloS one · 2021Article
- Monitoring of radiologic disease activity by serum neurofilaments in MS.Neurology(R) neuroimmunology & neuroinflammation · 2020Observational
- Prognostic value of serum neurofilaments in patients with clinically isolated syndromes.Neurology · 2019Article
- Neurofilament light chain serum levels correlate with 10-year MRI outcomes in multiple sclerosis.Annals of clinical and translational neurology · 2018Article
- Cytoskeleton as a Target of Quinolinic Acid Neurotoxicity: Insight from Animal Models.Molecular neurobiology · 2018Review
- Assembly and turnover of neurofilaments in growing axonal neurites.Biology open · 2018Article
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.Biology open · 2017Article
- Brain-Specific Cytoskeletal Damage Markers in Cerebrospinal Fluid: Is There a Common Pattern between Amyotrophic Lateral Sclerosis and Primary Progressive Multiple Sclerosis?International journal of molecular sciences · 2015Review
- The high molecular weight neurofilament subunit plays an essential role in axonal outgrowth and stabilization.Biology open · 2014Article
- Divergent and convergent roles for kinases and phosphatases in neurofilament dynamics.Journal of cell science · 2014Article
- Post-translational modifications of intermediate filament proteins: mechanisms and functions.Nature reviews. Molecular cell biology · 2014Review
- Local regulation of neurofilament transport by myelinating cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Charcot-Marie-Tooth causing HSPB1 mutations increase Cdk5-mediated phosphorylation of neurofilaments.Acta neuropathologica · 2013Article
- A neuronal death model: overexpression of neuronal intermediate filament protein peripherin in PC12 cells.Journal of biomedical science · 2012Article
- Article
- Physical model for the width distribution of axons.The European physical journal. E, Soft matter · 2009Article
- Microtubule-independent regulation of neurofilament interactions in vitro by neurofilament-bound ATPase activities.Biochemical and biophysical research communications · 2009Article
- Softness, strength and self-repair in intermediate filament networks.Experimental cell research · 2007Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the steady-state distribution and axonal transport of neurofilament (NF) subunits within growing axonal neurites of NB2a/d1 cells. Ultrastructural analyses demonstrated a longitudinally oriented "bundle" of closely apposed NFs that was surrounded by more widely spaced individual NFs. NF bundles were recovered during fractionation and could be isolated from individual NFs by sedimentation through sucrose. Immunoreactivity toward the restrictive C-terminal phospho-dependent antibody RT97 was significantly more prominent on bundled than on individual NFs. Microinjected biotinylated NF subunits, GFP-tagged NF subunits expressed after transfection, and radiolabeled endogenous subunits all associated with individual NFs before they associated with bundled NFs. Biotinylated and GFP-tagged NF subunits did not accumulate uniformly along bundled NFs; they initially appeared within the proximal portion of the NF bundle and only subsequently were observed along the entire length of bundled NFs. These findings demonstrate that axonal NFs are not homogeneous but, rather, consist of distinct populations. One of these is characterized by less extensive C-terminal phosphorylation and a relative lack of NF-NF interactions. The other is characterized by more extensive C-terminal NF phosphorylation and increased NF-NF interactions and either undergoes markedly slower axonal transport or does not transport and undergoes turnover via subunit and/or filament exchange with individual NFs. Inhibition of phosphatase activities increased NF-NF interactions within living cells. These findings collectively suggest that C-terminal phosphorylation and NF-NF interactions are responsible for slowing NF axonal transport.
Indexed as
Identifiers
11264295PMC6762414W2106807815What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.