ArticleAnalytical biochemistry2001
Optical determination of glutamine using a genetically engineered protein.
Article in Analytical biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 66 citations in OpenAlex.
- Studies of conformational changes of an arginine-binding protein from Thermotoga maritima in the presence and absence of ligand via molecular dynamics simulations with the coarse-grained UNRES force field.Journal of molecular modeling · 2015Article
- FIBS-enabled noninvasive metabolic profiling.Journal of visualized experiments : JoVE · 2014Article
- Periplasmic Binding Proteins in Thermophiles: Characterization and Potential Application of an Arginine-Binding Protein from Thermotoga maritima: A Brief Thermo-Story.Life (Basel, Switzerland) · 2013Review
- Article
- A biosensor for fluorescent determination of ADP with high time resolution.The Journal of biological chemistry · 2009Article
- Low-cost optical lifetime assisted ratiometric glutamine sensor based on glutamine binding protein.Analytical biochemistry · 2008Article
- Comparing the performance of the optical glucose assay based on glucose binding protein with high-performance anion-exchange chromatography with pulsed electrochemical detection: efforts to design a low-cost point-of-care glucose sensor.Journal of diabetes science and technology · 2007Article
- Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein.Protein science : a publication of the Protein Society · 2007Article
- Visualization of arginine influx into plant cells using a specific FRET-sensor.Journal of fluorescence · 2007Article
- Analysis of ligand binding to a ribose biosensor using site-directed mutagenesis and fluorescence spectroscopy.Protein science : a publication of the Protein Society · 2007Article
- Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor.Protein science : a publication of the Protein Society · 2005Article
- Construction of a fluorescent biosensor family.Protein science : a publication of the Protein Society · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a reagentless optical biosensor for glutamine based on the Escherichia coli glutamine binding protein (GlnBP). Site-directed mutagenesis was performed to engineer single cysteine mutants which were covalently modified with environmentally sensitive sulfhydryl-reactive probes. The fluorescence emission of acrylodan and 2-(4'-(iodoacetamido)anilino)naphthalene-6-sulfonic acid (IAANS) attached to GlnBP mutant S179C was shown to decrease 65 and 35%, respectively, upon titration with increasing amounts of glutamine (0 to 6.4 microM; K(Dapp) 160 nM). No significant changes in the fluorescence intensity were observed for the structurally similar amino acids glutamate, asparagine, and arginine. Time-resolved intensity decays showed a 2.4-fold decrease in mean lifetime for GlnBP S179C-acrylodan upon the addition of glutamine, indicating the possibility of a lifetime-based assay. Anisotropy decay measurements for GlnBPS179C-acrylodan showed a 13-ns rotational correlation time in the ligand-free state, whereas multiple correlation times were assigned in the glutamine-bound conformation. The decrease in fluorescence intensity of S179C-acrylodan was adapted to polarization sensing of glutamine. The engineered GlnBP is a first step toward the development of a nonenzymatic biosensor capable of determining glutamine concentrations in cell cultures.
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