ArticleMolecular and cellular biology2001
Two immunologically distinct human DNA polymerase alpha-primase subpopulations are involved in cellular DNA replication.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Switch from canonical to recombination-dependent DNA synthesis by M-CDK phosphorylation of DNA Polα.The EMBO journal · 2026Article
- Quantitative proteomics reveals novel protein interaction partners of PP2A catalytic subunit in pancreatic β-cells.Molecular and cellular endocrinology · 2016Article
- Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.Journal of diabetes research · 2016Article
- PP2A-B55β antagonizes cyclin E1 proteolysis and promotes its dysregulation in cancer.Cancer research · 2014Article
- Minichromosome maintenance 2 bound with retroviral Gp70 is localized to cytoplasm and enhances DNA-damage-induced apoptosis.PloS one · 2012Article
- Modification of histones by sugar β-N-acetylglucosamine (GlcNAc) occurs on multiple residues, including histone H3 serine 10, and is cell cycle-regulated.The Journal of biological chemistry · 2011Article
- Simian virus 40 activates ATR-Delta p53 signaling to override cell cycle and DNA replication control.Journal of virology · 2010Article
- Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.Nucleic acids research · 2009Article
- The human RNA polymerase II-associated factor 1 (hPaf1): a new regulator of cell-cycle progression.PloS one · 2009Article
- Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication.Molecular biology of the cell · 2007Article
- Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.Eukaryotic cell · 2005Article
- DNA replication: a complex matter.EMBO reports · 2003Review
- Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.Molecular and cellular biology · 2002Article
- Physical and functional interactions of the tumor suppressor protein p53 and DNA polymerase alpha-primase.Nucleic acids research · 2002Article
- PP2A as a master regulator of the cell cycle.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic labeling of primate cells revealed the existence of phosphorylated and hypophosphorylated DNA polymerase alpha-primase (Pol-Prim) populations that are distinguishable by monoclonal antibodies. Cell cycle studies showed that the hypophosphorylated form was found in a complex with PP2A and cyclin E-Cdk2 in G1, whereas the phosphorylated enzyme was associated with a cyclin A kinase in S and G2. Modification of Pol-Prim by PP2A and Cdks regulated the interaction with the simian virus 40 origin-binding protein large T antigen and thus initiation of DNA replication. Confocal microscopy demonstrated nuclear colocalization of hypophosphorylated Pol-Prim with MCM2 in S phase nuclei, but its presence preceded 5-bromo-2'-deoxyuridine (BrdU) incorporation. The phosphorylated replicase exclusively colocalized with the BrdU signal, but not with MCM2. Immunoprecipitation experiments proved that only hypophosphorylated Pol-Prim associated with MCM2. The data indicate that the hypophosphorylated enzyme initiates DNA replication at origins, and the phosphorylated form synthesizes the primers for the lagging strand of the replication fork.
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