ArticleMolecular and cellular biology2001
Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- TargetMITO: a rule-based model for generating functional synthetic mitochondrial targeting sequences in yeast.Nucleic acids research · 2026Article
- Ebselen restores mitochondrial function through metabolic rewiring in Barth syndrome and related mitochondrial diseases.EMBO molecular medicine · 2026Article
- Local mitochondrial physiology defined by mtDNA quality guides purifying selection.PLoS genetics · 2026Article
- Article
- The deubiquitinase Ubp3/Usp10 constrains glucose-mediated mitochondrial repression via phosphate budgeting.eLife · 2024Article
- Introducing carbon assimilation in yeasts using photosynthetic directed endosymbiosis.Nature communications · 2024Article
- The cytochromeLife science alliance · 2023Article
- Engineering artificial photosynthetic life-forms through endosymbiosis.Nature communications · 2022Article
- Yeast Mitochondrial Translation Initiation Factor 3 Interacts with Pet111p to PromoteInternational journal of molecular sciences · 2020Article
- Toward a Synthetic Yeast Endosymbiont with a Minimal Genome.Journal of the American Chemical Society · 2019Article
- Yeast mitochondrial protein Pet111p binds directly to two distinct targets inThe Journal of biological chemistry · 2019Article
- Plant Mitochondrial Inner Membrane Protein Insertion.International journal of molecular sciences · 2018Review
- The evolutionary history of Saccharomyces species inferred from completed mitochondrial genomes and revision in the 'yeast mitochondrial genetic code'.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Sounds of silence: synonymous nucleotides as a key to biological regulation and complexity.Nucleic acids research · 2013Review
- Yeast PPR proteins, watchdogs of mitochondrial gene expression.RNA biology · 2013Article
- Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome.Frontiers in physiology · 2013Article
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- Biogenesis and assembly of eukaryotic cytochrome c oxidase catalytic core.Biochimica et biophysica acta · 2012Review
- Multiple roles of the Cox20 chaperone in assembly of Saccharomyces cerevisiae cytochrome c oxidase.Genetics · 2012Article
- The carboxyl-terminal end of Cox1 is required for feedback assembly regulation of Cox1 synthesis in Saccharomyces cerevisiae mitochondria.The Journal of biological chemistry · 2010Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The mitochondrial gene encoding yeast cytochrome oxidase subunit II (Cox2p) specifies a precursor protein with a 15-amino-acid leader peptide. Deletion of the entire leader peptide coding region is known to block Cox2p accumulation posttranscriptionally. Here, we examined in vivo the role of the pre-Cox2p leader peptide and the mRNA sequence that encodes it in the expression of a mitochondrial reporter gene, ARG8m, fused to the 91st codon of COX2. We found within the coding sequence antagonistic elements that control translation: the positive element includes sequences in the first 14 codons specifying the leader peptide, while the negative element appears to be within codons 15 to 91. Partial deletions, point mutations, and local frameshifts within the leader peptide coding region were placed in both the cox2::ARG8m reporter and in COX2 itself. Surprisingly, the mRNA sequence of the first six codons specifying the leader peptide plays an important role in positively controlling translation, while the amino acid sequence of the leader peptide itself is relatively unconstrained. Two mutations that partially block translation can be suppressed by nearby sequence substitutions that weaken a predicted stem structure and by overproduction of either the COX2 mRNA-specific translational activator Pet111p or the large-subunit mitochondrial ribosomal protein MrpL36p. We propose that regulatory elements embedded in the translated COX2 mRNA sequence could play a role, together with trans-acting factors, in coupling regulated synthesis of nascent pre-Cox2p to its insertion in the mitochondrial inner membrane.
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