ArticleBiophysical journal2001
Voltage-induced nonconductive pre-pores and metastable single pores in unmodified planar lipid bilayer.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- [Coupling characteristics of cellular electroporation and electrodeformation based on dynamic Young's modulus: influence of membrane thickness].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2026Article
- The multiple sclerosis drug fingolimod induces pore-like membrane defects, contributing to its antimicrobial activity.The Journal of biological chemistry · 2026Article
- Multiple Sclerosis Drug Fingolimod Exhibits Antibacterial Activity through Bacterial Membrane Permeabilization.bioRxiv : the preprint server for biology · 2026Article
- Interleaflet Translocation of Second-Harmonic-Generation-Active Dye Molecules in Phospholipid Bilayers with Transmembrane Pores.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Methyl-β-cyclodextrin asymmetrically extracts phospholipid from bilayers, granting tunable control over differential stress in lipid vesicles.Soft matter · 2024Article
- Multiple conductance states of lipid pores during Voltage-Clamp electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP).International journal of molecular sciences · 2023Article
- Cellular Delivery of Large Functional Proteins and Protein-Nucleic Acid Constructs via Localized Electroporation.Nano letters · 2023Article
- Activation energy for pore opening in lipid membranes under an electric field.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Passive transport of CaBiophysical journal · 2023Article
- Effect of Cobalt Ferrite Nanoparticles in a Hydrophilic Shell on the Conductance of Bilayer Lipid Membrane.Membranes · 2022Article
- Modeling and simulation of current-clamp electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2022Article
- A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability.Scientific reports · 2022Article
- Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora's Box.International journal of molecular sciences · 2022Review
- Identification of electroporation sites in the complex lipid organization of the plasma membrane.eLife · 2022Article
- Latest developments on the mechanism of action of membrane disrupting peptides.Biophysics reports · 2021Article
- The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores.eLife · 2021Article
- Water Pores in Planar Lipid Bilayers at Fast and Slow Rise of Transmembrane Voltage.Membranes · 2021Article
- Clarification of electrical current importance in plasma gene transfection by equivalent circuit analysis.PloS one · 2021Article
- Free energy of hydrophilic and hydrophobic pores in lipid bilayers by free energy perturbation of a restraint.The Journal of chemical physics · 2020Article
19 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
Electric fields promote pore formation in both biological and model membranes. We clamped unmodified planar bilayers at 150-550 mV to monitor transient single pores for a long period of time. We observed fast transitions between different conductance levels reflecting opening and closing of metastable lipid pores. Although mean lifetime of the pores was 3 +/- 0.8 ms (250 mV), some pores remained open for up to approximately 1 s. The mean amplitude of conductance fluctuations (approximately 500 pS) was independent of voltage and close for bilayers of different area (40,000 and 10 microm(2)), indicating the local nature of the conductive defects. The distribution of pore conductance was rather broad (dispersion of approximately 250 pS). Based on the conductance value and its dependence of the ion size, the radius of the average pore was estimated as approximately 1 nm. Short bursts of conductance spikes (opening and closing of pores) were often separated by periods of background conductance. Within the same burst the conductance between spikes was indistinguishable from the background. The mean time interval between spikes in the burst was much smaller than that between adjacent bursts. These data indicate that opening and closing of lipidic pores proceed through some electrically invisible (silent) pre-pores. Similar pre-pore defects and metastable conductive pores might be involved in remodeling of cell membranes in different biologically relevant processes.
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