ArticleBiophysical journal2001
Lateral organization and domain formation in a two-component lipid membrane system.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- High-Resolution Ultrasound Spectroscopy for the Determination of Phospholipid Transitions in Liposomal Dispersions.Pharmaceutics · 2022Article
- Phospholipase A activity of adenylate cyclase toxin mediates translocation of its adenylate cyclase domain.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered.Scientific reports · 2017Article
- MRI-guided monitoring of thermal dose and targeted drug delivery for cancer therapy.Pharmaceutical research · 2013Review
- Membrane restructuring by phospholipase A2 is regulated by the presence of lipid domains.Biophysical journal · 2011Article
- Room temperature stabilization of oral, live attenuated Salmonella enterica serovar Typhi-vectored vaccines.Vaccine · 2011Article
- The anti-inflammatory drug indomethacin alters nanoclustering in synthetic and cell plasma membranes.The Journal of biological chemistry · 2010Article
- Effect of indomethacin on bile acid-phospholipid interactions: implication for small intestinal injury induced by nonsteroidal anti-inflammatory drugs.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Membrane microheterogeneity: Förster resonance energy transfer characterization of lateral membrane domains.European biophysics journal : EBJ · 2010Review
- Article
- A novel fluorescent probe that senses the physical state of lipid bilayers.Biophysical journal · 2009Article
- Macroscopic domain formation during cooling in the platelet plasma membrane: an issue of low cholesterol content.Biochimica et biophysica acta · 2009Article
- Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative.Biophysical journal · 2009Article
- Absence of fluid-ordered/fluid-disordered phase coexistence in ceramide/POPC mixtures containing cholesterol.Biophysical journal · 2006Article
- Domain-induced activation of human phospholipase A2 type IIA: local versus global lipid composition.Biophysical journal · 2006Article
- Detection of submicron-sized raft-like domains in membranes by small-angle neutron scattering.The European physical journal. E, Soft matter · 2005Article
- Domain formation in phosphatidylinositol monophosphate/phosphatidylcholine mixed vesicles.Biophysical journal · 2004Article
- Temperature dependence of the surface topography in dimyristoylphosphatidylcholine/distearoylphosphatidylcholine multibilayers.Biophysical journal · 2004Article
- Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.Biophysical journal · 2003Article
- Time-resolved fluorescence and fourier transform infrared spectroscopic investigations of lateral packing defects and superlattice domains in compositionally uniform cholesterol/phosphatidylcholine bilayers.Biophysical journal · 2003Article
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5 authors.
Funding
Abstract
The thermodynamic phase behavior and lateral lipid membrane organization of unilamellar vesicles made from mixtures of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2 distearoyl-sn-glycero-3-phosphocholine (DSPC) were investigated by fluorescence resonance energy transfer (FRET) as a function of temperature and composition. This was done by incorporating a headgroup-labeled lipid donor (NBD-DPPE) and acceptor (N-Rh-DPPE) in low concentrations into the binary mixtures. Two instances of increased energy transfer efficiency were observed close to the phase lines in the DMPC/DSPC phase diagram. The increase in energy transfer efficiency was attributed to a differential preference of the probes for dynamic and fluctuating gel/fluid coexisting phases. This differential preference causes the probes to segregate (S. Pedersen, K. Jørgensen, T. R. Baekmark, and O. G. Mouritsen, 1996, Biophys. J. 71:554-560). The observed increases in energy transfer match with the boundaries of the DMPC/DSPC phase diagram, as measured by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). We propose that the two instances of probe segregation are due to the presence of DMPC-rich and DSPC-rich domains, which form a dynamic structure of gel/fluid coexisting phases at two different temperatures. Monitoring the melting profile of each lipid component independently by FTIR shows that the domain structure is formed by DMPC-rich and DSPC-rich domains rather than by pure DMPC and DSPC domains.
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