ArticleBiophysical journal2001
Cell volume kinetics of adherent epithelial cells measured by laser scanning reflection microscopy: determination of water permeability changes of renal principal cells.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Single-file transport of water through membrane channels.Faraday discussions · 2018Article
- Culturing primary rat inner medullary collecting duct cells.Journal of visualized experiments : JoVE · 2013Article
- Rapid aquaporin translocation regulates cellular water flow: mechanism of hypotonicity-induced subcellular localization of aquaporin 1 water channel.The Journal of biological chemistry · 2012Article
- Monitoring single-channel water permeability in polarized cells.The Journal of biological chemistry · 2011Article
- The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites.Biophysical journal · 2010Article
- Routes of epithelial water flow: aquaporins versus cotransporters.Biophysical journal · 2010Article
- Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy.Pflugers Archiv : European journal of physiology · 2010Article
- Modelling the swelling assay for aquaporin expression.Journal of mathematical biology · 2008Article
- Contribution of aquaporins to cellular water transport observed by a microfluidic cell volume sensor.Analytical chemistry · 2008Article
- Asymmetry in the osmotic response of a rat cortical collecting duct cell line: role of aquaporin-2.The Journal of membrane biology · 2005Article
- Effect of dDAVP on basolateral cell surface water permeability in the outer medullary collecting duct.European biophysics journal : EBJ · 2003Article
- Cyclic AMP is sufficient for triggering the exocytic recruitment of aquaporin-2 in renal epithelial cells.EMBO reports · 2003Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The water channel aquaporin-2 (AQP2), a key component of the antidiuretic machinery in the kidney, is rapidly regulated by the antidiuretic hormone vasopressin. The hormone exerts its action by inducing a translocation of AQP2 from intracellular vesicles to the cell membrane. This step requires the elevation of intracellular cyclic AMP. We describe here a new method, laser scanning reflection microscopy (LSRM), suitable for determining cellular osmotic water permeability coefficient changes in primary cultured inner medullary collecting duct (IMCD) cells. The recording of vertical-reflection-mode x-z-scan section areas of unstained, living IMCD cells proved useful and valid for the investigation of osmotic water permeability changes. The time-dependent increases of reflection-mode x-z-scan section areas of swelling cells were fitted to a single-exponential equation. The analysis of the time constants of these processes indicates a twofold increase in osmotic water permeability of IMCD cells after treatment of the cells both with forskolin, a cyclic AMP-elevating agent, and with Clostridium difficile toxin B, an inhibitor of Rho proteins that leads to depolymerization of F-actin-containing stress fibers. This indicates that both agents lead to the functional insertion of AQP2 into the cell membrane. Thus, we have established a new functional assay for the study of the regulation of the water permeability at the cellular level.
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