ArticleEMBO reports2001
Chromosomal DNA demethylation specified by protein binding.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- APC Promoter Hypermethylation as a Prognostic Marker in Breast Cancer Patients.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- GC skew is a conserved property of unmethylated CpG island promoters across vertebrates.Nucleic acids research · 2015Article
- Human lymphoid translocation fragile zones are hypomethylated and have accessible chromatin.Molecular and cellular biology · 2015Article
- Article
- Transcriptional activity affects the H3K4me3 level and distribution in the coding region.Molecular and cellular biology · 2010Article
- VEZF1 elements mediate protection from DNA methylation.PLoS genetics · 2010Article
- The temperature-dependent change in methylation of the Antirrhinum transposon Tam3 is controlled by the activity of its transposase.The Plant cell · 2006Article
- CDX1 is an important molecular mediator of Barrett's metaplasia.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Site-selective in vivo targeting of cytosine-5 DNA methylation by zinc-finger proteins.Nucleic acids research · 2003Article
- The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Reversal of methylation-mediated repression with short-chain fatty acids: evidence for an additional mechanism to histone deacetylation.Nucleic acids research · 2001Article
- Protein binding protects sites on stable episomes and in the chromosome from de novo methylation.Molecular and cellular biology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the present study, we utilize the well-characterized Escherichia coli lac repressor/operator system to demonstrate that protein binding can lead to demethylation at the binding sites in the chromosome. Similar to the findings using the episome, we found that the presence of LacI in the cells can lead to demethylation of methylated lacO in the chromosome and the LacI inhibitor, isopropyl-beta-D-thiogalactopyranoside (IPTG), can prevent demethylation of the methylated lacO. The lacO sites become progressively more demethylated over time with the presence of LacI, supporting the role of protein occupancy in demethylation targeting. These results validate our earlier conclusions using a stable episomal system, and establish for the first time that protein binding can specify sites of demethylation in the chromosome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.