ArticleEMBO reports2000
Genome-wide insertional mutagenesis in human cells by the Drosophila mobile element Minos.
Article in EMBO reports, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature.Bioresources and bioprocessing · 2022Article
- Article
- Regulation of DNA transposition by CpG methylation and chromatin structure in human cells.Mobile DNA · 2013Article
- Mariner transposons as genetic tools in vertebrate cells.Genetica · 2009Review
- Passport, a native Tc1 transposon from flatfish, is functionally active in vertebrate cells.Nucleic acids research · 2009Article
- Transposon tools hopping in vertebrates.Briefings in functional genomics & proteomics · 2008Review
- Pigs taking wing with transposons and recombinases.Genome biology · 2007Review
- Transposons for cancer gene discovery: Sleeping Beauty and beyond.Genome biology · 2007Review
- The DNA transposon Minos as a tool for transgenesis and functional genomic analysis in vertebrates and invertebrates.Genome biology · 2007Review
- Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.Genome biology · 2007Review
- Article
- Article
- The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes.Genetics · 2004Article
- The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells.Nucleic acids research · 2003Article
- Generating and manipulating transgenic animals using transposable elements.Reproductive biology and endocrinology : RB&E · 2003Review
- Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The medaka fish Tol2 transposable element can undergo excision in human and mouse cells.Journal of human genetics · 2003Article
- A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- In vivo transposition of Minos, a Drosophila mobile element, in mammalian tissues.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Regulated transposition of a fish transposon in the mouse germ line.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of efficient non-viral methodologies for genome-wide insertional mutagenesis and gene tagging in mammalian cells is highly desirable for functional genomic analysis. Here we describe transposon mediated mutagenesis (TRAMM), using naked DNA vectors based on the Drosophila hydei transposable element Minos. By simple transfections of plasmid Minos vectors in HeLa cells, we have achieved high frequency generation of cell lines, each containing one or more stable chromosomal integrations. The Minos-derived vectors insert in different locations in the mammalian genome. Genome-wide mutagenesis in HeLa cells was demonstrated by using a Minos transposon containing a lacZ-neo gene-trap fusion to generate a HeLa cell library of at least 10(5) transposon insertions in active genes. Multiple gene traps for six out of 12 active genes were detected in this library. Possible applications of Minos-based TRAMM in functional genomics are discussed.
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