ArticleThe Journal of cell biology2001
Internal modification of U2 small nuclear (sn)RNA occurs in nucleoli of Xenopus oocytes.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- Unveiling Small Non-Coding RNA Dynamics During Recombinant Adeno-Associated Virus Production.Biotechnology journal · 2025Article
- Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq.Nucleic acids research · 2022Article
- "Lost and Found": snoRNA Annotation in the Xenopus Genome and Implications for Evolutionary Studies.Molecular biology and evolution · 2020Article
- scaRNAs and snoRNAs: Are they limited to specific classes of substrate RNAs?RNA (New York, N.Y.) · 2019Article
- Base editing: precision chemistry on the genome and transcriptome of living cells.Nature reviews. Genetics · 2018Review
- Nucleoli cytomorphology in cutaneous melanoma cells - a new prognostic approach to an old concept.Diagnostic pathology · 2017Article
- RNA modification in Cajal bodies.RNA biology · 2017Review
- Review
- Structure and Organization of the Engraulidae Family U2 snRNA: An Evolutionary Model Gene?Journal of molecular evolution · 2015Article
- Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes.RNA (New York, N.Y.) · 2014Article
- The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Pearls are novel Cajal body-like structures in the Xenopus germinal vesicle that are dependent on RNA pol III transcription.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.The EMBO journal · 2011Article
- Cajal body surveillance of U snRNA export complex assembly.The Journal of cell biology · 2010Article
- Regulation of pre-mRNA splicing in Xenopus oocytes by targeted 2'-O-methylation.RNA (New York, N.Y.) · 2010Article
- Small Cajal body-specific RNAs of Drosophila function in the absence of Cajal bodies.Molecular biology of the cell · 2009Article
- In search of nonribosomal nucleolar protein function and regulation.The Journal of cell biology · 2009Review
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.Molecular and cellular biology · 2008Article
- Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.Molecular and cellular biology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
U2 small nuclear (sn)RNA contains a large number of posttranscriptionally modified nucleotides, including a 5' trimethylated guanosine cap, 13 pseudouridines, and 10 2'-O-methylated residues. Using Xenopus oocytes, we demonstrated previously that at least some of these modified nucleotides are essential for biogenesis of a functional snRNP. Here we address the subcellular site of U2 internal modification. Upon injection into the cytoplasm of oocytes, G-capped U2 that is transported to the nucleus becomes modified, whereas A-capped U2 that remains in the cytoplasm is not modified. Furthermore, by injecting U2 RNA into isolated nuclei or enucleated oocytes, we observe that U2 internal modifications occur exclusively in the nucleus. Analysis of the intranuclear localization of fluorescently labeled RNAs shows that injected wild-type U2 becomes localized to nucleoli and Cajal bodies. Both internal modification and nucleolar localization of U2 are dependent on the Sm binding site. An Sm-mutant U2 is targeted only to Cajal bodies. The Sm binding site can be replaced by a nucleolar localization signal derived from small nucleolar RNAs (the box C/D motif), resulting in rescue of internal modification as well as nucleolar localization. Analysis of additional chimeric U2 RNAs reveals a correlation between internal modification and nucleolar localization. Together, our results suggest that U2 internal modification occurs within the nucleolus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.