ArticleJournal of biomedical materials research2001
Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system.
Article in Journal of biomedical materials research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- Soft bioreactor systems: a necessary step toward engineered MSK soft tissue?Frontiers in robotics and AI · 2024Review
- Synthetic Calcium-Phosphate Materials for Bone Grafting.Polymers · 2023Review
- Evaluation of Polymeric Particles for Modular Tissue Cultures in Developmental Engineering.International journal of molecular sciences · 2023Article
- Differentiation of human induced pluripotent stem cells into functional lung alveolar epithelial cells in 3D dynamic culture.Frontiers in bioengineering and biotechnology · 2023Article
- A Compilation of Study Models for Dental Pulp Regeneration.International journal of molecular sciences · 2022Review
- Article
- Modular microcarrier technologies for cell-based bone regeneration.Journal of materials chemistry. B · 2020Review
- Review
- An Air Bubble-Isolating Rotating Wall Vessel Bioreactor for Improved Spheroid/Organoid Formation.Tissue engineering. Part C, Methods · 2019Article
- Three-dimensional in vitro modeling of malignant bone disease recapitulates experimentally accessible mechanisms of osteoinhibition.Cell death & disease · 2018Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- Osteogenic differentiation of encapsulated rat mesenchymal stem cells inside a rotating microgravity bioreactor: in vitro and in vivo evaluation.Cytotechnology · 2018Article
- MC3T3 infiltration and proliferation in bovine trabecular scaffold regulated by dynamic flow bioreactor and augmented by low-intensity pulsed ultrasound.Journal of orthopaedic translation · 2018Article
- Article
- Effect of Rotation on Scaffold Motion and Cell Growth in Rotating Bioreactors.Tissue engineering. Part A · 2017Article
- Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers.BMC biotechnology · 2015Article
- Advances in cell culture: anchorage dependence.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications.Journal of tissue engineering · 2015Article
- Three-dimensional extracellular matrix scaffolds by microfluidic fabrication for long-term spontaneously contracted cardiomyocyte culture.Tissue engineering. Part A · 2014Article
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A novel approach was utilized to grow in vitro mineralized bone tissue using lighter-than-water, polymeric scaffolds in a high aspect ratio rotating bioreactor. We have adapted polymer microencapsulation methods for the formation of hollow, lighter-than-water microcarriers of degradable poly(lactic-co-glycolic acid). Scaffolds were fabricated by sintering together lighter-than-water microcarriers from 500 to 860 microm in diameter to create a fully interconnected, three-dimensional network with an average pore size of 187 microm and aggregate density of 0.65 g/mL. Motion in the rotating bioreactor was characterized by numerical simulation and by direct measurement using an in situ particle tracking system. Scaffold constructs established a near circular trajectory in the fluid medium with a terminal velocity of 98 mm/s while avoiding collision with the bioreactor wall. Preliminary cell culture studies on these scaffolds show that osteoblast-like cells readily attached to microcarrier scaffolds using controlled seeding conditions with an average cell density of 6.5 x 10(4) cells/cm(2). The maximum shear stress imparted to attached cells was estimated to be 3.9 dynes/cm(2). In addition, cells cultured in vitro on these lighter-than-water scaffolds retained their osteoblastic phenotype and showed significant increases in alkaline phosphatase expression and alizarin red staining by day 7 as compared with statically cultured controls.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.