ArticleJournal of immunology (Baltimore, Md. : 1950)2001
NF-kappa B p50-dependent in vivo footprints at Ig S gamma 3 DNA are correlated with mu-->gamma 3 switch recombination.
Article in Journal of immunology (Baltimore, Md. : 1950), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Impaired B cell development and function in the absence of IkappaBNS.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Switch region identity plays an important role in Ig class switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Sgamma3 switch sequences function in place of endogenous Sgamma1 to mediate antibody class switching.The Journal of experimental medicine · 2008Article
- Expression of AID transgene is regulated in activated B cells but not in resting B cells and kidney.Molecular immunology · 2008Article
- Activation-induced deaminase-mediated class switch recombination is blocked by anti-IgM signaling in a phosphatidylinositol 3-kinase-dependent fashion.Molecular immunology · 2008Article
- Identification of murine B cell lines that undergo somatic hypermutation focused to A:T and G:C residues.European journal of immunology · 2008Article
- Article
- NF-kappa B binds to the immunoglobulin S gamma 3 region in vivo during class switch recombination.European journal of immunology · 2006Article
- AID-dependent histone acetylation is detected in immunoglobulin S regions.The Journal of experimental medicine · 2006Article
- Shifts in targeting of class switch recombination sites in mice that lack mu switch region tandem repeats or Msh2.The Journal of experimental medicine · 2005Article
- Mapping of a functional recombination motif that defines isotype specificity for mu-->gamma3 switch recombination implicates NF-kappaB p50 as the isotype-specific switching factor.The Journal of experimental medicine · 2004Article
- Class-switch recombination: after the dawn of AID.Current opinion in immunology · 2003Review
- Two new isotype-specific switching activities detected for Ig class switching.Journal of immunology (Baltimore, Md. : 1950) · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
NF-kappa B has been demonstrated to play critical roles in multiple aspects of immune responses including Ig H chain isotype switching. To better define the specific roles the p50 subunit of NF-kappa B plays in mu-->gamma 3 switch recombination (SR), we systematically evaluated p50-deficient B cells for activities that are strongly correlated with SR. B cell activation with LPS plus anti-IgD-dextran plus IL-5 plus IL-4 plus TGF-beta produced normal levels of proliferation and gamma3 germline transcripts in p50-deficient B cells, but mu-->gamma 3 SR was impaired. In vitro binding studies previously showed that NF-kappa B p50 homodimer binds the switch nuclear B-site protein (SNIP) of the S gamma 3 tandem repeat. Ligation-mediated PCR in vivo footprint analysis demonstrates that the region spanning the SNIP and switch nuclear A-site protein (SNAP) binding sites of the S gamma 3 region are contacted by protein in normal resting splenic B cells. B cells that are homozygous for the targeted disruption of the gene encoding p50 (-/-) show strong aberrant footprints, whereas heterozygous cells (+/-) reveal a partial effect in S gamma 3 DNA. These studies provide evidence of nucleoprotein interactions at switch DNA in vivo and suggest a direct interaction of p50 with S gamma 3 DNA that is strongly correlated with SR competence.
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