ArticleInfection and immunity2001
Antimycobacterial agent based on mRNA encoding human beta-defensin 2 enables primary macrophages to restrict growth of Mycobacterium tuberculosis.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 85 citations in OpenAlex.
- Vitamin D induces interleukin-1β expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection.PLoS pathogens · 2013Trial
- Peptide-Based Strategies AgainstPharmaceuticals (Basel, Switzerland) · 2025Review
- Irisin alleviated sepsis via enhancing macrophage phagocytosis and reducing inflammation levels.Frontiers in immunology · 2025Article
- Synthetic mRNA delivered to human cells leads to expression of Cpl-1 bacteriophage-endolysin with activity againstMolecular therapy. Nucleic acids · 2024Article
- Review
- Lung epithelial cells interact with immune cells and bacteria to shape the microenvironment in tuberculosis.Thorax · 2022Review
- Diminished Systemic and Mycobacterial Antigen Specific Anti-microbial Peptide Responses in Low Body Mass Index-Latent Tuberculosis Co-morbidity.Frontiers in cellular and infection microbiology · 2020Article
- Microdeletion on chromosome 8p23.1 in a familial form of severe Buruli ulcer.PLoS neglected tropical diseases · 2018Article
- Article
- Immuno-Stimulatory Peptides as a Potential Adjunct Therapy against Intra-Macrophagic Pathogens.Molecules (Basel, Switzerland) · 2017Review
- Host Antimicrobial Peptides: The Promise of New Treatment Strategies against Tuberculosis.Frontiers in immunology · 2017Review
- Design, synthesis and structure-activity relationship study of wollamide B; a new potential anti TB agent.PloS one · 2017Article
- Expressions of Antimicrobial Peptides LL-37, Human Beta Defensin-2 and -3 in the Lesions of Cutaneous Tuberculosis and Tuberculids.Chinese medical journal · 2016Article
- Defensins: The Case for Their Use against Mycobacterial Infections.Journal of immunology research · 2016Review
- Air pollution particulate matter alters antimycobacterial respiratory epithelium innate immunity.Infection and immunity · 2015Article
- Antimycobacterial activity of Pichia pastoris-derived mature bovine neutrophil β-defensins 5.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Augmentation of epithelial resistance to invading bacteria by using mRNA transfections.Infection and immunity · 2013Article
- Article
- Antimicrobial Peptides in Innate Immunity against Mycobacteria.Immune network · 2011Article
- [Study of the antitumor activity of alveolar macrophages after transfected human INF-γ gene].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Human macrophages are hosts for Mycobacterium tuberculosis, the causative agent of tuberculosis, which killed approximately 1.87 million people in 1997. Human alveolar macrophages do not express alpha- or beta-defensins, broad-spectrum antimicrobial peptides which are expressed in macrophages from other species more resistant to infection with M. tuberculosis. It has been previously reported that M. tuberculosis is susceptible to killing by defensins, which may explain the difference in resistance. Defensin peptides have been suggested as a possible therapeutic strategy for a variety of infectious diseases, but development has been hampered by difficulties in their large-scale production. Here we report the cellular synthesis of human beta-defensin 2 via highly efficient mRNA transfection of human macrophages. This enabled mycobactericidal and mycobacteristatic activity by the macrophages. Although human macrophages are difficult to transfect with plasmid vectors, these studies illustrate that primary macrophages are permissive for mRNA transfection, which enabled expression of a potentially therapeutic protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.